3D Cell Model Imaging & Analysis

3D cell model imaging and analysis workflows provide more physiologically relevant insights but are not without their challenges. 

High-content assays using 3D objects such as cysts or organoids can be challenging from the perspectives of both image acquisition and image analysis. 

Multicellular 3D “oids” (tumoroids, spheroids, organoids) have the potential to better predict the effects of drug candidates during preclinical screening. 

Explore our Technical Note unveiling a robust workflow for advanced cytotoxicity analysis in 3D cell cultures.

In drug discovery programs, multicellular spheroids have emerged as powerful tools to bridge the gap between in vitro cell culture models and in vivo tissues. 

Researchers are increasingly looking to 3D cell cultures, microtissues, and organoids to bridge the gap between 2D cell cultures and in vivo animal models. 

Automated image acquisition and analysis of tissue sections can be challenging owing to the inherent height variations throughout each specimen, and this can hinder their use in screening applications.

3D cell cultures offer a more physiologically relevant option, but there are currently only a few high throughput screening (HTS) 3D systems that have high consistency and easily allow integration with downstream analysis methods.

Spinning disk confocal microscopy is a common tool for live cell microscopy and reduces background fluorescence from out of focus planes.