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High-content Screening Research Areas

High-content analysis (HCA) has expanded into all corners of life science since its beginnings in drug discovery and is now used widely in a diverse range of scientific research areas including oncology, neuroscience, infectious disease, and toxicology, to name just a few.

HCA merges the benefits of high-throughput automation and unbiased analysis with microscopic imaging. The combination of high sample throughput with multiparametric imaging and multivariate analysis enables the detection of even subtle phenotypic changes which isn’t possible with traditional laboratory techniques.

Discover how high-content analysis and screening can advance your research in a selection of areas.

For research use only. Not for use in diagnostic procedures.

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Cancer research

Cellular imaging has revolutionized cancer biology by enabling cells to be seen more clearly and in more detail as well as creating meaningful functional assays for the development of drugs which prevent tumor growth and metastasis.

Cellular imaging has revolutionized cancer biology by enabling cells to be seen more clearly and in more detail as well as creating meaningful functional assays for the development of drugs which prevent tumor growth and metastasis.

Cancer is a class of diseases in which a group of cells display new properties, such as hyperactive growth and division, protection against programmed cell death, loss of respect for normal tissue boundaries, and the ability to become established in diverse tissue environments.

Cancer research is an intense scientific effort to understand and discover disease processes and discover possible therapies. Advanced cellular imaging techniques allow us to visualize important aspects of cancer including tumor cell mobility, invasion, metastasis and angiogenesis.

Scientists can distinguish host cells from a tumor with single-cell resolution. Visualization of many aspects of cancer initiation and progression in vivo is now possible, providing greater context and more accuracy to cancer research.

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Neuroscience

High content analysis is a powerful tool for neuroscience that provides rich data for research including assays that can quantify various aspects of dendritic trees, protein aggregation, transcription factor translocation, neurotransmitter receptor internalization, neuron and synapse number, cell migration, proliferation and apoptosis.

High content analysis is a powerful tool for neuroscience that provides rich data for research including assays that can quantify various aspects of dendritic trees, protein aggregation, transcription factor translocation, neurotransmitter receptor internalization, neuron and synapse number, cell migration, proliferation and apoptosis.

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Infectious diseases

HCS plays a significant role in infectious disease research as it enables high throughput functional and phenotypic assays that can be adapted to a wide range of pathogens including virus, bacteria and eukaryotic parasites.

HCS plays a significant role in infectious disease research as it enables high throughput functional and phenotypic assays that can be adapted to a wide range of pathogens including virus, bacteria and eukaryotic parasites.

Applications include genetic siRNA interference screens for identifying host factors involved in host–pathogen interactions, but also screens for lead discovery in drug discovery. High-content analysis enables, for example, intracellular tracking of viral particles to profile the antiviral mechanisms of each compound and sensitive measurements of bacterial infection rates. Adaptation to high-throughput screening in bacteriology and parasitology has already led to the discovery of new types of host-specific inhibitors that differ from those inhibitors that act directly on microbes.

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Toxicity

Undesired toxic side effects are still amongst the most common reasons for failure of new chemical entities during late stage drug development and can even lead to costly withdrawals of approved drugs from the market. 

Undesired toxic side effects are still amongst the most common reasons for failure of new chemical entities during late stage drug development and can even lead to costly withdrawals of approved drugs from the market. 

It is therefore necessary to assess compounds in the early phases of the discovery process reliably for potential geno-, hepato-, nephro and cardiotoxic liabilities in order to reduce development costs and increase research efficiency. High content screening is a cost effective tool to run dedicated toxicity tests on relevant model systems.

Such dedicated assays include micronuclei assays to identify genotoxic effects, cell health studies on heptatocytes and kidney cells, advanced assays on liver microtissues, or using primary human cells, hypertrophy studies on cardiomyocytes.

But even functional drug discovery HCS assays can provide important information on potential unwanted properties of a compound in addition to the primary effect readout.

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Stem cell research

High-content analysis is a powerful technology to analyze stem cell differentiation efficiently under a large variety of conditions and over long periods of times. Scientists are able visualize and analyze complex cellular processes, identify and characterize cells in their various stages of development.

High-content analysis is a powerful technology to analyze stem cell differentiation efficiently under a large variety of conditions and over long periods of times. Scientists are able visualize and analyze complex cellular processes, identify and characterize cells in their various stages of development.

Stem cell research has the potential to make a huge impact on a wide range of human health problems. Stem cell research could facilitate the development of new treatments for human diseases such as diabetes and Parkinson’s, aid the treatment and prevention of medical conditions resulting from abnormal cell division and cell differentiation, and enable the testing of new drugs without the need for animals or human testers. It could also provide a renewable source of replacement cells and tissues to treat diseases such as Alzheimer's disease, stroke, heart disease, osteoarthritis and rheumatoid arthritis.

For this fast paced area of research, Revvity provides state-of-the-art high content screening solutions to stem cell researchers all over the world. Our dedicated team of HCS specialists have an in-depth understanding of the imaging-based stem cell applications and will help you choose the right solution for your research requirements.

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3D Analysis of Cell Invasion using Operetta
high-content screening 3D cell analysis cell invasion
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3D Volumetric Analysis of Luminal Spaces Inside Cysts or Organoids
High-content screening high-content analysis 3D 3D cell model organoid cyst 3D analysis
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3D volumetric and zonal analysis of solid spheroids
high-content screening 3D high-content screening spheroids 3D cell culture analysis
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Analysis of mitochondrial dynamics in human iPSC-derived neurons using the Operetta CLS High-Content Analysis System
mitochondria mitochondrial dynamics human iPSC-derived neurons live-cell imaging high-content screening
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Artificial intelligence, machine learning and deep learning: applications in cellular imaging for improved drug discovery productivity
Applications of artificial intelligence AI Cellular imaging Applications of machine learning Applications of deep learning
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Automated Single Cell Tracking using Operetta
High-content screening Single Cell Tracking automated single cell tracking chemokinesis live cell assay
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Cell painting - a cellular imaging and machine learning approach to drug discovery
cell painting cell painting solutions cell painting workflow cellular imaging solutions
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Cell painting for phenotypic screening
cell painting cell painting solutions cell painting workflow cell painting assay setup cellular imaging solutions
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Product Info
Cell painting: from images to innovation
Cell Painting Phenotypic screening Fluorescent dyes cell painting workflow high-content screening
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FAQ
Cell painting: your questions answered
cell painting assays cell painting assay cell painting guidance best practices for cell painting
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Technical Note
Clearing strategies for 3D spheroids
high-content screening 3D high-content screening spheroids 3D cell culture analysis spheroid clearing
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Complete Virology Solutions Infographic
Virology Solutions Detection Neuralization Testing Infectious Disease
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Cytotoxicity studies on 3D primary liver microtissues
Cellular imaging technologies Evaluation of hepatotoxicity Liver toxicity testing Hepatotoxicity assay procedure High content Imaging
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Cytotoxicity Studies on Live Primary Human Hepatocytes
High-content screening live cells primary hepatocytes live-cell cytotoxicity analysis
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Fast kinetic calcium flux imaging using the Opera Phenix Plus High-Content Screening System
Fetal fraction
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High content screening in three dimenstions
3D HCS 3D high-content screening high-content imaging high-content analysis imaging in 3D
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Technical Note
How to perform long term live cell imaging in a high‑content analysis system
Live-cell imaging long term imaging live-cell moitoring live cells
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Literature - Publication Review
Imaging and gene editing approaches highlight important role of autophagy in controlling Mtb infection.
High-content imaging CRISPR-Cas 9 gene editing TB infection autophagy
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Improved high-content imaging of tissue sections
tissue section tissue section imaging high-content screening
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Case Study
Improving the throughput of a neuroprotection assay using the opera phenix high content screening system
neuroscience neuroprotection assay Opera Phenix high-content screening system high-content screening
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Kinetic analysis of calcium flux activity in human iPSC-derived neurons using the Opera Phenix Plus system
Fast kinetic imaging high-content screening calcium flux, iPSC-derived neurons
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Label-free analysis of cardiomyocyte beating using the Opera Phenix Plus System
cardiomyocyte cardiotoxic assessment cardiotoxicity quantification of cardiotoxicity
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Literature - Publication Review
Morphological Profiling of Human T and NK Lymphocytes by High-Content Imaging
high-content imaging morphological profiling T and NK lymphocytes cell therapy immunotherapy
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Opera Phenix High-Content Screening System Technical Performance: Speed
high-content screening high-content analysis Opera Phenix Synchrony optics water immersion objectives water immersion lens phenotypic screening speed
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Opera Phenix High-Content Screening System: Improved 3D imaging
3D cell models 3D imaging Spheroids organoids 3D Cell model imaging HCS with 3D cells
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