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High Content Screening Instruments

High Content Screening (HCS) and High Content Analysis (HCA) products

High-Content Analysis (HCA) or High-Content Screening (HCS) combines high-throughput automated imaging and analysis to extract quantitative multi-parametric data at the single-cell level. Originally developed as a complementary technology to traditional biochemical high-throughput screening (HTS) in drug discovery, today high-content screening is established in a far broader area of the life science space as an unbiased imaging method to assess cellular function.

High-Content Imaging (HCI) is typically defined as lower throughput automated microscopy (<100,000 samples or data points) compared to high-content screening. However, high-content imaging is often used interchangeably with the terms high-content screening and high-content analysis.

High-content screening offers a number of advantages over traditional techniques:
  • More information from cellular samples
    Fully automated imaging and unbiased quantitative image analysis exploits the full potential of microscopy, improving your lab’s throughput and productivity.
  • More meaningful results
    Screening can be performed in more physiologically and biologically relevant systems than in classical biochemical assays.
  • More questions answered simultaneously
    Data sets are more information-rich compared to non-imaging read-outs, allowing a number of questions to be answered simultaneously.

(1) Buchser, William, et al. "Assay development guidelines for image-based high content screening, high content analysis and high content imaging." Assay guidance manual [Internet] (2014).

For research use only. Not for use in diagnostic procedures.

HCS Cell Image of Month

Explore our high-content screening system technologies

Confocal imaging
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Spinning disk confocal microscopy offers several advantages over conventional optical microscopy, including widefield and laser scanning confocal microscopy.

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Water immersion objectives
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Proprietary automated water-immersion objectives with very high numerical aperture deliver and capture more photons and provide a higher resolution in XYZ than conventional objectives.

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Illumination
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Stability, brightness and spectral fit for excitation are key considerations for illumination sources in fluorescent imaging.

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sCMOS cameras
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The large-format sCMOS camera – one in Operetta CLS or up to four of them in the Opera Phenix - delivers low noise, wide dynamic range, and high resolution – perfect for sensitive and quantitative measurements at short exposure times.

Intelligent image acquisition
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More accurately target your object of interest for significantly reduced acquisition and analysis times with intelligent acquisition technology for HCS.

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Machine learning
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Machine learning techniques enable supervised and unsupervised feature selection and clustering of highly multi-parametric data sets.

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Explore our high-content screening system products

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Operetta CLS high content analysis system

Operetta® CLS™ is a high throughput microplate imager for High-Content Analysis (HCA). It can acquire, analyze and manage fluorescence, brightfield and digital phase contrast images.

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Operetta® CLS™ is a high throughput microplate imager for High-Content Analysis (HCA). It can acquire, analyze and manage fluorescence, brightfield and digital phase contrast images.

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Opera phenix plus high-content screening system

The Opera Phenix® Plus high-content screening system is the premier confocal solution for today's most demanding high content applications.

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The Opera Phenix® Plus high-content screening system is the premier confocal solution for today's most demanding high content applications.

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High-content screening system filters and lenses

Enable sensitive imaging on our cellular vivo imaging systems - at whatever resolution you desire. With our custom-designed optics, including optical filters, objectives and zoom lenses, you have the ideal view for any application.

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Enable sensitive imaging on our cellular vivo imaging systems - at whatever resolution you desire. With our custom-designed optics, including optical filters, objectives and zoom lenses, you have the ideal view for any application.

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Meet our users

Read how scientists around the globe are using our high-content screening products and services to advance their research in a variety of fields.

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Read how scientists around the globe are using our high-content screening products and services to advance their research in a variety of fields.

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Please note that product labeling (such as kit insert, product label, and kit box) may be different compared to the company branding. Please contact your local representative for further details.