Imaging just got smarter with the Opera Phenix OptIQ System
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High content imaging provides a greater depth of information than other approaches, allowing you to explore cellular processes in detail.
Quantify in detail
Analyze phenotypic changes at scale, from single cells to entire populations. High-content imaging empowers detailed quantification.
Enhance 3D imaging
Water immersion objectives elevate 3D image quality, revealing intricate details within complex biological structures.
Boost throughput
Add more cameras to increase speed and efficiency. High-content imaging accelerates your research pipeline.
Applications
Microphysiological Systems (MPS)
Deep understanding of 3D cell models with clearer images that reveal critical features and processes.
Functional genomic screening
Create genetic evidence with ease by phenotyping cellular response to thousands of gene manipulations in a single experiment.
Cell painting
Simplify multiparametric screening with out of the box reagents and analysis to reduce complexity (and create more time for you to focus on the science).
Drug sensitivity testing
Determine rational treatment selections by rapidly characterizing cellular function in response to drug treatment.
Fast response assays
Capture rapid cellular events and dynamic processes with fast frame rates and on-board liquid dispensing.
Hypoxic environment applications
Capture cellular images in low oxygen concentrations using the onboard environmental control chamber allowing cells to be imaged in a more realistic physiological condition.
Opera Phenix OptIQ family: typical configurations
Opera Phenix OptIQ Single
The same sensitivity and resolution as the rest of the Phenix family, with the ability to upgrade later with additional cameras.
Opera Phenix OptIQ Simultaneous
Higher speed, dual-camera system for multi-color, simultaneous confocal image acquisition and fast multiplexing.
Opera Phenix OptIQ FRET
With its five lasers and four-camera setup, it supports CFP/YFP FRET applications to map protein-protein interactions.
Opera Phenix OptIQ Screener
With advanced throughput and performance, it delivers four cameras and four higher powered lasers - supporting screening of large libraries.
Configuration details
| Single | Simultaneous | FRET | Screener | ||
|---|---|---|---|---|---|
| Lasers | 375/425 nm | Unavailable | Optional | ✓ | Unavailable |
| 405 nm | ✓ | ✓ | Optional | ✓ | |
| 488 nm | ✓ | ✓ | ✓ | ✓ | |
| 561 nm | ✓ | ✓ | ✓ | ✓ | |
| 640 nm | ✓ | ✓ | ✓ | ✓ | |
| System options | Number of cameras (confocal) | 1 | 2 | 4 | 4 |
| Environmental control | Optional | Optional | ✓ | Optional | |
| Water immersion lenses | ✓ | ✓ | ✓ | ✓ | |
| Transmitted light | ✓ | ✓ | ✓ | ✓ | |
| On-board liquid handling | Optional | Optional | Optional | Optional | |
| Robotics / Automation compatible | ✓ | ✓ | ✓ | ✓ | |
| Imaging modes | Minimal crosstalk imaging | ✓ | ✓ | ✓ | ✓ |
| Ratiometric FRET | Suitable | Suitable | Suitable (CFP/YFP) | ||
| 3D imaging | ✓ | ✓ | ✓ | ✓ | |
| Multi-color fluorescence imaging | ✓ | ✓ | ✓ | ✓ | |
| Multi-color simultaneous confocal imaging | Unavailable | ✓ | ✓ | ✓ | |
| Brightfield and digital phase contrast | ✓ | ✓ | ✓ | ✓ | |
| Fluorescence widefield imaging | ✓ | ✓ | ✓ | ✓ | |
| Fast frame rate imaging | ✓ | ✓ | ✓ | ✓ |
Image acquisition and analysis simplified with Harmony software
Intuitive workflow
Harmony software offers an intuitive user interface that guides you from image acquisition to analysis and evaluation.
Templates for quick set-up
Templates allow you to set up acquisition channels and parameters efficiently.
Ready-made solutions
Choose from pre-built solutions for common image analysis tasks, simplifying your workflow.
Customizable building blocks
Create, configure, and customize your own high-content analysis applications using image analysis building blocks.
Advanced features
Harmony includes advanced analysis capabilities, such as texture and STAR morphology analysis, providing detailed descriptions of cellular morphology and robust differentiation of phenotypes.
Data management
The software automatically stores analysis results and metadata, including assay layout, instrument settings, and user-defined keywords and annotations.
AI-based image analysis
Using pre-trained deep-learning image-analysis models, Phenologic.AI allows for segmentation and classification of cells in brightfield and fluorescent images for easier analysis of live and fixed cell assays.
Progress faster with our verified solutions
Microplates for high-content imaging
Utilize PhenoPlate™ 384-well microplates designed to support optimal performance in high-content imaging applications. Employ CellCarrier Spheroid ULA plates for imaging 3D models.
PhenoVue cellular imaging reagents
Ready-to-use kits and reagents with straightforward protocols. Extensively tested to provide optimal formulations and long-term stability.
Automation and workstations
Improve throughput, productivity, and reduce variability and reagent costs. Benefit from automated workflows using the explorer™ G3 workstations for cell painting, 3D cell culture, or phenotypic screening.
Efficient data management
Export results automatically into the Image Artist™ platform that uses high performance computing and an industry standard object store to provide a scalable, multi-user solution for image analysis and management.
Product information
Overview
Opera Phenix OptIQ high-content screening system for your most demanding high-content applications.
- Enhanced speed using a dual - or four-camera configuration with simultaneous imaging.
- Synchrony Optics™ combines a microlens-enhanced Nipkow spinning disk with a pinhole distance optimized for thick and 3D samples.
- Dual-view excitation of neighboring spectral channels minimizes crosstalk.
- Custom-designed high-NA water immersion objectives capture more photons and provide high-image resolution even in thick samples.
- Fast imaging frame rate of up to 100 fps and optional pipettor module captures fast cellular responses.
Key features
- Multiple cameras - Increase imaging speed by using up to four high-quantum efficiency cameras (>95% QE) and simultaneous multi-color acquisition, especially for extensive stacks for 3D models.
- Microlens-enhanced spinning disk confocality – A pinhole and a microlens disk spinning in tandem for increased light efficiency. Pinhole distances suited for 3D samples, reducing out-of-focus light.
- Automated water-immersion objectives - Improve image quality and get better data by enhancing the signal and improving the z resolution while capturing more light.
- Proprietary Synchrony Optics - Reduced spectral crosstalk during simultaneous acquisition of multiple colors by separating adjacent fluorescence excitation and emission patterns, providing greater speed and higher sensitivity.
- Machine learning - Easily create algorithms without being an image analysis expert using our Phenologic proprietary machine-learning technology.
- Intelligent image acquisition - Image only the objects you are interested in, centered 3D models at high magnification, thereby reducing imaging time and data size.
- Powerfully simple analysis - Harmony high-content imaging and analysis software can make you more productive faster with ready-made templates and simple steps to a custom analysis.
- 3D analysis - Explore your cell models by visualizing them in a 3D- and an XYZ-viewer and quantify volumetric and other 3D related phenotypic readouts.
- Find Organoids building block - The Find Organoids building block within Harmony software can detect organoids in brightfield stacks using 2D or 3D detection with improved object splitting.
Resources
Opera Phenix OptIQ high-content screening system flyer
Improved brightfield image segmentation of organoids in Harmony and Image Artist analysis software
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