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Water Immersion Objectives

Proprietary automated water-immersion objectives with very high numerical aperture deliver and capture more photons and provide a higher resolution in XYZ than conventional objectives – in fact, they capture up to four times more light than high numerical aperture air objectives.

You can benefit in two ways: Delicate live-cell samples can be excited with less light to protect them from photodamage or you can significantly increase the throughput of applications such as 3D stack acquisition.

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Water immersion objective

Comparison of images of acquired on Operetta CLS using either an air lens (40x) or a water immersion lens (40x).

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HeLa cells labeled with Hoechst (nuclei, blue), anti-a-tubulin-AlexaFluor® 488 (tubulin, green) and TRITC-Phalloidin (actin, orange). Images were acquired using the same exposure settings and are displayed with the same contrast settings. Left: Air objective. Right: Water immersion objective.

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3D InSight™ Human Liver Microtissues from InSphero labeled with Hoechst (nuclei, blue) and CellMask™ Deep Red plasma membrane stain (membranes, red). Left: Air objective. Right: Water immersion objective.

For research use only. Not for use in diagnostic procedures.