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LANCE TR-FRET Assays

LANCE® (lanthanide chelate excite) TR-FRET technology combines the benefits of Time Resolved Fluorescence (TRF) with those of Förster Resonance Energy Transfer (FRET) in a simple, highly-sensitive, and highly reproducible assay platform for detection, quantitation and screening in microplate format.

The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays. Furthermore, FRET detection utilizes energy donor and energy acceptor fluorophore labels in solution to create a no-wash detection assay. By combining the benefits of TRF and FRET, LANCE TR-FRET offers a multitude of benefits over standard fluorometric detection:

  • Homogeneous assay format (no wash steps, no separation steps)
  • Reduced background leading to better S:B
  • Large stoke’s shift
  • Wider dynamic range
  • Extended signal stability
  • Increased sensitivity. Lower detection limits of 10 pg/mL compared to 100 pg/mL for standard fluorometric detection
  • Faster time to results

From biomarker detection assays, to cAMP/GPCR studies, to kinase and epigenetic assays, our LANCE and LANCE Ultra TR-FRET assays are sure to save you time and hassle.

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HTRF (homogeneous time resolved technology)

We now also offer Revvity HTRF® assay technology. Find out more about HTRF (Homogeneous Time Resolved Technology) and the products offered in this technology here.

We now also offer Revvity HTRF® assay technology. Find out more about HTRF (Homogeneous Time Resolved Technology) and the products offered in this technology here.

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Biochemical assays

Biochemical assays remain an important tool for the drug discovery process. Whether you need a binding assay, kinase assay, other enzymatic assay, or would like to build your own assay utilizing our Toolbox reagents, LANCE has a variety of biochemical assays available.

Biochemical assays remain an important tool for the drug discovery process. Whether you need a binding assay, kinase assay, other enzymatic assay, or would like to build your own assay utilizing our Toolbox reagents, LANCE has a variety of biochemical assays available.

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Assays for biomarker detection

Biomarker detection is key for advancing precision medicine. An array of biomarker detection kits are available utilizing our LANCE technologies in therapeutic areas including:

Biomarker detection is key for advancing precision medicine. An array of biomarker detection kits are available utilizing our LANCE technologies in therapeutic areas including:

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Assays for cell signaling

Breakthrough discoveries in cell signaling and cell signal transduction are coming from laboratories that maximize high-quality results while minimizing hassles. And LANCE helps you do just that. With a wide selection of phospho- and total protein detection kits available, we are here to help you better understand the intracellular pathways of phosphorylated proteins.

Breakthrough discoveries in cell signaling and cell signal transduction are coming from laboratories that maximize high-quality results while minimizing hassles. And LANCE helps you do just that. With a wide selection of phospho- and total protein detection kits available, we are here to help you better understand the intracellular pathways of phosphorylated proteins.

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LANCE custom services

Are you working on a novel target? If you are looking to develop your own assay Revvity custom labeling services can label your assay components with LANCE donor and acceptor molecules. Or maybe you are you short on time and need an assay developed quickly? Custom LANCE assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our custom team.

Are you working on a novel target? If you are looking to develop your own assay Revvity custom labeling services can label your assay components with LANCE donor and acceptor molecules. Or maybe you are you short on time and need an assay developed quickly? Custom LANCE assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our custom team.

block6 LANCE page How can we help you
How can we help you?

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays Revvity is here to help you succeed. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays Revvity is here to help you succeed. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development. They are available to assist in both assay development and troubleshooting.

Application support knowledgebase (ASK): Need help at 1am? We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more.

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Developing a LANCE TR-FRET assay for screening PD-1 and PD-L1 binding inhibitors.
lance tr-fret pd1 pd-1 pdl1 inhibitors assays
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LANCE Ultra TR-FRET-based detection and modulation of phosphorylated STAT3 levels in human cells.
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Measurement of p38/MAPK Activity Using LANCE
lance p38 mapk assays kinase
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