Skip to main content
Menu

HTRF™ (Homogeneous Time Resolved Fluorescence) is a no-wash technology that combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence.

This technology can be utilized in cellular or biochemical assays, employing competitive and non-competitive formats. It may be used  for drug discovery, biotherapy and basic research in therapeutic areas such as oncology, CNS and metabolic and immune disorders. Researchers can explore a wide range of applications, including GPCRs, kinases, epigenetics, biotherapy, and quantification of a range of biomarkers and cytokines.

  • Easy to perform: homogeneous assay with no wash requirement
  • High level of sensitivity, signal stable in time
  • Time and labor savings, rapid and accurate results
  • Amenable to miniaturization
  • Ability to detect and analyze small and large complexes with low to high affinity interactions
  • Field of applications: basic research, target discovery and validation, HTS and primary screening, secondary screening, lead optimization 

For research use only. Not for use in diagnostic procedures.
 

Hero-image-872x872

Explore our solutions

Target Area

Target area

Explore cell-based and biochemical assays by target area of interest, including cell and gene therapy, GPCRs, targeted protein degradation, and more.

Explore cell-based and biochemical assays by target area of interest, including cell and gene therapy, GPCRs, targeted protein degradation, and more.

  • Biologics screening and characterization: Explore assays for biologics screening, mechanism-of-action studies, and biomanufacturing.
  • Biomarker detection: Accurate detection and quantitation of molecular targets across serum, plasma, culture supernatants, cellular lysates, and other sample types.
  • Cell and gene therapy: Discover and advance cell and gene therapy research with bioanalytical immunoassays for CGT development.
  • Cell signaling: Focus on cell signaling and intracellular phosphorylated proteins, with streamlined approaches to pathway analysis.
  • Cytokines: Access a comprehensive range of cytokine assay kits, including interleukins, interferons, chemokines, tumor necrosis factors, and lymphokines.
  • Epigenetics: Leverage our epigenetics toolbox, designed to detect specific methylation and acetylation marks on histone peptide substrates.
  • GPCRs: Measure cAMP levels and track downstream protein phosphorylation with functional GPCR assays.
  • Protein kinases: Explore an extensive portfolio of protein phosphorylation assays, available in cell-based or biochemical no-wash formats.
  • Protein-protein interactions: Utilize highly sensitive assays to study protein interactions involved in a range of biological processes.
  • Receptor binding: Access cell-based solutions with no separation steps and eliminate radioactive waste, an improvement over traditional SPA and radioligand binding assays.
  • Targeted protein degradation: Monitor protein expression levels and characterize the mechanism of action using our total and phospho kits.
Therapeutic Area

Therapeutic area

Explore cell-based and biochemical assays by your therapeutic area of research, including oncology, neuroscience, metabolic diseases, and more.

Explore cell-based and biochemical assays by your therapeutic area of research, including oncology, neuroscience, metabolic diseases, and more.

  • Autoimmune diseases: Comprehensive reagents for autoimmune disease research, including rheumatoid arthritis, lupus, inflammatory bowel disease, multiple sclerosis, and more.
  • Obesity, diabetes, and metabolic diseases: Solutions for drug discovery in obesity, diabetes, and metabolic diseases, featuring assays for biomarker detection, protein analysis, and GPCR research.
  • Fibrosis: Ready-to-use assays for monitoring biomarkers, phosphoproteins, and transcription factors relevant to fibrotic disease research.
  • Immuno-oncology: Assays for studying immunity and immune cells, covering protein, biomarker, and cytokine detection, as well as cell toxicity and proliferation.
  • Oncology: A comprehensive oncology portfolio, including assays for cytokines, biomarkers, phospho-proteins, cell proliferation, cytotoxicity, and more.
  • Neuroscience: A wide range of neuroscience assays to support research in neurodegenerative disorders such as Alzheimer’s, Parkinson’s, and Huntington's disease.
  • Virology: Assay technologies for viral studies, enabling assessment of viral life cycles and antiviral immune responses with streamlined workflows.

Featured resources

bg-element-listing-page bg-element-listing-page-mobile
Products & services
Resource library