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Virology Reagents

Viruses and virally transmitted diseases have been impacting human health and well-being for millennia. Some of our greatest scientific challenges and medical triumphs have involved virology, from smallpox to HIV and so many others. And today is no different. With the current outbreak of the novel coronavirus, SARS-CoV-2, and its corresponding viral disease, COVID-19, virology is once again in the spotlight.

Revvity’s range of reagents offer support throughout the research and drug discovery process. Whether you are assessing stages of the viral life cycle or monitoring an antiviral immune response, our assay technologies provide simple, sensitive, streamlined workflows to help accelerate your viral studies.

Viral life cycle

From viral antigen and protein:protein interaction assays, to cytotoxicity and dsRNA kits, Alpha, HTRF®, and luminescence technologies offer solutions for identifying viral presence, attachment, and genome integration, as well as monitoring cytotoxicity and cell death.

Antiviral immune response

Part of developing immunity, and therefore a critical component to successful vaccination, is the body’s inflammatory response. As such, cytokines, inflammatory pathways and inflammatory mediators are important factors to look at when monitoring immunity. Pathways such as MAP kinase, JAK/STAT, and NFκB play a central role in the antiviral immune response along with ligands such as IL-6, TNFα, and the INFs (-γ, -α, -β, and -λ). Revvity offers a wide range of Alpha and HTRF cytokine detection kits, phosphorylation detection kits, and toolbox reagents to monitor these critical indicators of the viral immune response.

COVID-19

Reportedly, a cytokine storm has been associated with COVID-19 infection1, leading to the rise in cytokine panel assays being used when studying the SARS-CoV-2 virus. These cytokine storms are the result of the body over-expressing multiple cytokines in a short period of time in an effort to protect itself from the virus, due to pro-inflammatory loops. This over inflammation is more harmful than helpful to the body. Revvity offers Alpha and HTRF kits for the detection of IL-6, TNFα, and signaling proteins within the JAK/STAT pathway, all of which have shown to play an important role in this cytokine storm.

In addition to a range of cytokines, C-reactive protein (CRP) is also a protein of interest in the study of COVID-19. An inflammatory marker produced by the liver; CRP has been found to rise with the progression of COVID-19. Revvity offers a no-wash, AlphaLISA® detection assay for the quantification of CRP in serum, buffered solution, or cell culture medium.

1Pedersen SF, Ho YC. SARS-CoV-2: a storm is raging. J Clin Invest. 2020 Apr 13. pii: 137647. doi: 10.1172/JCI137647. [Epub ahead of print]

block1 Alpha
Alpha technology

Alpha is a homogeneous versatile, bead-based platform that enables you to assay even the most complex samples in one well and with no wash steps.

Alpha is a homogeneous versatile, bead-based platform that enables you to assay even the most complex samples in one well and with no wash steps.

A range of AlphaLISA detection kits are available for virology studies. Available kits allow for virus quantification, and cytokine detection. Specifically, the AlphaLISA IL-6 and C Reaction Protein (CRP) kits are of particular interest in looking at the COVID-19 cytokine storm. Additionally, AlphaLISA kits and Toolbox Acceptor Beads are available for immunoglobulin detection.

Alpha SureFire kits can be used to assess both innate and adaptive antiviral immunity. When assessing innate antiviral immunity a range of SureFire kits are available for proteins in the TLR, cGAS-STING, TLR/ cGAS-STING/ Inflammasome/ NF-kB, and JAK/STAT pathways. Kits are also available for assessing adaptative antiviral immunity, both through the T-cell activation pathway and the B-cell activation pathway.

HTRF block 2
HTRF

HTRF assays are fast, sensitive, homogeneous, and ready to use with no wash steps.

HTRF assays are fast, sensitive, homogeneous, and ready to use with no wash steps.

A range of HTRF kits are available for viral dsRNA and cytokine detection. Specifically, the HTRF IL-6 kit isof particular interest in looking at the COVID-19 cytokine storm.

HTRF assays can be used to assess both innate and adaptive antiviral immunity. When assessing innate antiviral immunity a range of HTRF assays are available for proteins in the TLR, cGAS-STING, TLR/ cGAS-STING/ Inflammasome/ NF-kB, and JAK/STAT pathways. Assays are also available for assessing adaptative antiviral immunity, both through the T-cell activation pathway and the B-cell activation pathway.

Furthermore, viral neutralization assays utilizing HTRF technology are also available.

block 3 virology reagents
Luminescence assays

Reporter gene, cytotoxicity, and cell proliferation assays are important tools not only in virology studies, but in drug discovery in general. Luminescence assays are ideal for use in viral neutralization assays and to monitor cell viability.

Reporter gene, cytotoxicity, and cell proliferation assays are important tools not only in virology studies, but in drug discovery in general. Luminescence assays are ideal for use in viral neutralization assays and to monitor cell viability.

Luminescence detection platforms provide simple, reproducible results over a wide dynamic range. Our luciferase-based luminescence assays include reporter gene and ATP-monitoring assays.

On Point Block 4
Custom assay development

Are you working on a novel target? If you are looking to develop your own assay Revvity custom labeling services can custom conjugate your antibody or biomolecule to any bead, or biotinylate or DIG-label your targets. Or maybe you are you short on time and need an assay developed quickly? Custom Alpha and HTRF assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our custom team.

Are you working on a novel target? If you are looking to develop your own assay Revvity custom labeling services can custom conjugate your antibody or biomolecule to any bead, or biotinylate or DIG-label your targets. Or maybe you are you short on time and need an assay developed quickly? Custom Alpha and HTRF assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our custom team.

See Revvity’s customer development and labeling pages for more information on HTRF customization

Assay Development
Custom Conjugation Labeling

how can we help you
How can we help you?

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays Revvity is here to help you succeed. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays Revvity is here to help you succeed. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development. They are available to assist in both assay development and troubleshooting.

Application support knowledgebase (ASK): Need help at 1am? We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more. Accessible 24 hours a day, 7 days a week on our website.

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A Comparative Study of Two Immunoassay Platforms to Determine Lentivirus Titer for CAR-T Development

<p>The main vectors of gene therapy in research are viruses. The most popular tool for gene delivery is a genetically modified...</p>

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Alpha SureFire Ultra no-wash immunoassay catalog

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ATPlite assay performance in human primary cells.

In this application note three ATP monitoring luminescence assays were assessed and compared head-to-head: (i) ATPlite™ from...

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Avoiding biotin interference in AlphaLISA assays

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Biotin-Free AlphaLISA Assays for the Detection of Cytokines in PBMC Supernatants

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Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...

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Comparison of EMT Biomarker Expression in 2D Monolayer and 3D Spheroid Cultures in a Prostate Cancer

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NIH NCATS case study: using accelerated screening research for SARS-CoV-2 drug repurposing candidates

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Proliferation and cell death analyses of 3D cultures using Revvity CellCarrier Spheroid ULA microplates and ATPlite 3D products.

In the process of developing new therapeutic molecules, toxicity – whether it is to be avoided or whether it is desired – is an...

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Protein extraction using the Omni Bead Ruptor Elite with detection of phosphorylation events using AlphaLISA no-wash assays

Use this application note to learn how to combine protein extraction using the Omni Bead Ruptor Elite bead mill homogenizer with...

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Rapid, No-wash Measurement of Immune Checkpoint Molecules and Cytokines in Co-cultures of Immune Cells and Cancer Cell Lines

Breast cancer tumors can adapt to immune cell infiltration by responding to the increased concentration of interferon gamma (IFN-ɣ...

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Reagent solutions for autoimmunity research.

Advance your autoimmune disease research and benefit from Revvity broad offering of reagent technologies

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Tracking Inflammasome Activity via Measuring IL-1β levels with AlphaLISA and HTRF

<p>It has been suggested that inflammation promotes malignancy via proinflammatory cytokines, such as IL-1ß, which enhance immune...</p>

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