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Epigenetic Research Reagents

The epigenetics’ field revolutionized the understanding of heredity. While heredity has long been considered a consequence of alterations or mutations in DNA sequences carried over to the next generation, we now know that epigenetics also involves hereditary changes in gene expression. These changes are caused by alterations in gene expression regulated by changes in chromosomes, DNA accessibility or chromatin structure, and do not affect the DNA sequence.

Epigenetic changes can be caused by DNA methylation, post-translational modification of histone proteins or non-coding actions of RNA in the nucleus.

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Read on for a concise summary of three epigenetic modifications and as Revvity assays and reagents that can help you in your discovery journey.

As the understanding of the role of epigenetic changes in various disease states advances, the need for high quality reagents that can rapidly identify and validate therapeutic molecules that target these enzymes is increasing. Over the years, Revvity has built a world-class platform to study epigenetics. With a growing portfolio of no-wash assays available in cell-based or biochemical formats, scientists can efficiently address over a dozen histone methylation and acetylation states, as well as rely on a rich toolbox of reagent to assemble custom epigenetics assays.

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DNA methylation

DNA methylation is the main type of epigenetic modification that regulates gene expression and maintains the stability of the genome. It is the process by which a methyl group (CH3) is added to the C5 position of cytosines. This occurs mainly where a cytosine is followed by a guanine (CpG sites) in a DNA sequence, although non-cCpG methylation occurs in plants and sometimes in mammals.

DNA methylation is the main type of epigenetic modification that regulates gene expression and maintains the stability of the genome. It is the process by which a methyl group (CH3) is added to the C5 position of cytosines. This occurs mainly where a cytosine is followed by a guanine (CpG sites) in a DNA sequence, although non-cCpG methylation occurs in plants and sometimes in mammals.

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Histone modification

Epigenetic gene regulation also is controlled by changes in histones that make up the nucleosome and by histone modification. Histones are a family of proteins with a key role in the organization and regulation of DNA. They exist in five types (H1, H2A, H2B, H3, and H4) and act as spools that DNA can wrap around in a condensed chromatin state.

Epigenetic gene regulation also is controlled by changes in histones that make up the nucleosome and by histone modification. Histones are a family of proteins with a key role in the organization and regulation of DNA. They exist in five types (H1, H2A, H2B, H3, and H4) and act as spools that DNA can wrap around in a condensed chromatin state.

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Noncodings RNA

Expression of noncoding RNAs also plays a role in epigenetic gene regulation. Non-coding RNAs (ncRNAs) are RNA strands that do not code for protein sequences and are not translated into proteins, even though they are transcribed from DNA like all other RNA. Rather, they fulfill an array of functions related to the regulation of cellular processes.

Expression of noncoding RNAs also plays a role in epigenetic gene regulation. Non-coding RNAs (ncRNAs) are RNA strands that do not code for protein sequences and are not translated into proteins, even though they are transcribed from DNA like all other RNA. Rather, they fulfill an array of functions related to the regulation of cellular processes.

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