Automated HMW DNA Extraction
Automated high molecular weight DNA extraction for archiving and long read sequencing
Extraction of High Molecular Weight (HMW) DNA (> 100 kb) is currently often performed with manual, time-consuming, and costly methods. As manual workflows are subject to risk of human error and confirm differences in operator handling or laboratory conditions, more time and effort are often required to confirm experimental findings. In worst case scenarios, integrity of samples may compromised.
The chemagic™ nucleic acid purification systems automate the extraction of HMW DNA, improving consistency of results while ensuring sample integrity at higher throughputs and faster turnaround times. Compared with other automated platforms, the chemagic system provided higher DNA yields of good purity alongside a significantly greater HMW DNA extraction efficiency. The chemagic systems have been used successfully in conjunction with long read sequencing technologies from Oxford Nanopore2,4,5,6 and PacBio1,3. The greater HMW DNA extraction efficiency afforded by chemagic technology also makes it the choice of many biobanks worldwide that require long-term storage stability for archiving and a nucleic acid quality that can be applied to diverse molecular assays2,7,8.
Webinars:
For research use only. Not for use in diagnostic procedures.
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Customer experiences
Automated DNA extraction methods are revolutionizing genetic research, as demonstrated by these two insightful webinars. Karine Auribault from Paris and Emily Farrow from Kansas will share their experiences implementing high-throughput DNA isolation techniques in hospital settings. These presentations underscore the critical importance of efficient (HMW) DNA extraction in enabling a wide range of genetic tests and assays, from MLPA and Sanger sequencing to cutting-edge long read sequencing.
Automated DNA extraction methods are revolutionizing genetic research, as demonstrated by these two insightful webinars. Karine Auribault from Paris and Emily Farrow from Kansas will share their experiences implementing high-throughput DNA isolation techniques in hospital settings. These presentations underscore the critical importance of efficient (HMW) DNA extraction in enabling a wide range of genetic tests and assays, from MLPA and Sanger sequencing to cutting-edge long read sequencing.
Suggested links:
Webinar review: High quality DNA for genetic testing
In the ever-evolving field of genetic testing, the webinar titled “High Quality DNA with Revvity’s chemagic 360 for Genetic Testing” presented by Dr. Karine Auribault, a seasoned expert in molecular genetics and diagnostics, offered valuable insights.
Unleashing the power of genomics: A webinar review
Watch this on-demand webinar to learn how the chemagic nucleic acid purification workflow can support your HMW DNA analysis.
HMW DNA extraction for long read sequencing
Learn more about efficient HMW DNA isolation in our Application Note. We compared extraction performance from blood based on DNA yield, purity, and integrity between two automated nucleic acid extraction systems.
Learn more about efficient HMW DNA isolation in our Application Note. We compared extraction performance from blood based on DNA yield, purity, and integrity between two automated nucleic acid extraction systems.
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Comparison of automated nucleic acid purification systems HMW DNA extraction efficiency
With automation being increasingly employed in laboratories performing nucleic acid purification, a more consistent quality of nucleic acids is generally expected.
Biobanking
Drawing on extensive expertise in high-throughput extractions across diverse sample types such as blood, buffy coat, swabs, and saliva, we have established a global presence in biobanking. Our chemagic nucleic acid isolation ensures archive-quality specimens, and our ability to automate entire workflows, from primary sample handling to downstream QC and assay setup, has positioned us as a trusted collaborator in various population genetic cohort studies.
Drawing on extensive expertise in high-throughput extractions across diverse sample types such as blood, buffy coat, swabs, and saliva, we have established a global presence in biobanking. Our chemagic nucleic acid isolation ensures archive-quality specimens, and our ability to automate entire workflows, from primary sample handling to downstream QC and assay setup, has positioned us as a trusted collaborator in various population genetic cohort studies.
Our contributions extend to notable initiatives like the "All of Us Research Programme" from the Mayo Clinic7, the German National Cohort/NAKO Gesundheitsstudie8, and the British Initiative, including the 100,000 Genomes Project, among others.
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Dried blood spots for advanced DNA sequencing
Dried blood spots (DBS) are widely utilized in newborn screening, large population-based surveys, and biobanking due to their minimal blood volume requirements, ease of collection, and convenient transportation and storage. However, extracting high-quality DNA from DBS for next-generation sequencing (NGS) has posed significant challenges. This application note presents Revvity’s optimized workflow for targeted sequencing of DNA extracted from DBS, which addresses these challenges effectively.
Automated nucleic acid isolation fulfilling biobanking needs
Biobanking core laboratories and biospecimen repositories are facing the demanding task to extract high-quality DNA and RNA for long-term storage from diverse sample materials like whole blood, buffy coats, and fresh, frozen or fixed tissues.
High quality DNA isolation suitable for ultra rapid sequencing
A major challenge of using Whole-Genome Sequencing (WGS) for high-throughput genomic analysis is the amount of time required to perform the entire procedure from DNA isolation from the primary samples to analysis of the sequencing results.
Whole genome sequencing with Oxford Nanopore
Discover how a leading laboratory enhanced their Oxford Nanopore ligation-based whole genome sequencing workflow by incorporating chemagic-purified high molecular weight DNA extraction.
This optimization demonstrates the critical impact of upstream sample preparation on downstream sequencing quality and data integrity.
Discover how a leading laboratory enhanced their Oxford Nanopore ligation-based whole genome sequencing workflow by incorporating chemagic-purified high molecular weight DNA extraction.
This optimization demonstrates the critical impact of upstream sample preparation on downstream sequencing quality and data integrity.
High molecular weight DNA extraction advances
Read this article on Biocompare which examines breakthrough automated high molecular weight (HMW) DNA extraction technologies that preserve DNA fragments over 100 kb while reducing processing time from hours to minutes. The key innovation involves transitioning from traditional silica membranes to automated magnetic bead-based systems like chemagic™, which enable gentler handling and reduced DNA shearing essential for long-read sequencing applications. These advances particularly benefit challenging samples such as FFPE tissues and forensic materials, while supporting the growing demands of personalized medicine and clinical diagnostics requiring high-quality genomic data.
Read this article on Biocompare which examines breakthrough automated high molecular weight (HMW) DNA extraction technologies that preserve DNA fragments over 100 kb while reducing processing time from hours to minutes. The key innovation involves transitioning from traditional silica membranes to automated magnetic bead-based systems like chemagic™, which enable gentler handling and reduced DNA shearing essential for long-read sequencing applications. These advances particularly benefit challenging samples such as FFPE tissues and forensic materials, while supporting the growing demands of personalized medicine and clinical diagnostics requiring high-quality genomic data.
Genome-wide methylation detection and episignature analysis using PacBio long-read sequencing
In a study published in Genome Medicine (2026), Radboud University Medical Center researchers achieved genome-wide 5-methylcytosine detection using PacBio long-read sequencing, powered by high-quality DNA extraction with the chemagic DNA Blood 4K kit. Analyzing 30 whole-blood samples, the team successfully verified methylation patterns across 25 imprinted genomic regions and distinguished KMT2A-related Wiedemann-Steiner syndrome individuals from controls. chemagic's consistent extraction of High Molecular Weight DNA (HMW DNA) from archived blood samples proved essential for reliable long-read methylation profiling and allele-specific pattern detection, advancing functional genomic testing for rare developmental disorders and next-generation epigenetic research.
In a study published in Genome Medicine (2026), Radboud University Medical Center researchers achieved genome-wide 5-methylcytosine detection using PacBio long-read sequencing, powered by high-quality DNA extraction with the chemagic DNA Blood 4K kit. Analyzing 30 whole-blood samples, the team successfully verified methylation patterns across 25 imprinted genomic regions and distinguished KMT2A-related Wiedemann-Steiner syndrome individuals from controls. chemagic's consistent extraction of High Molecular Weight DNA (HMW DNA) from archived blood samples proved essential for reliable long-read methylation profiling and allele-specific pattern detection, advancing functional genomic testing for rare developmental disorders and next-generation epigenetic research.
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References:
- Lang K, Wagner I, Schöne B, et al. ABO allele-level frequency estimation based on population-scale genotyping by next generation sequencing. BMC Genomics. 2016;17:374. Published 2016 May 20.
- Beyter D, Ingimundardottir H, Oddsson A, et al. Long-read sequencing of 3,622 Icelanders provides insight into the role of structural variants in human diseases and other traits. Nature Genetics. 2021 Jun;53(6):779-786.
- Steiert TA, Fuß J, Juzenas S, et al. High-throughput method for the hybridisation-based targeted enrichment of long genomic fragments for PacBio third-generation sequencing. NAR Genom Bioinform. 2022;4(3):lqac051. Published 2022 Jul 13.
- Schmidt J, Berghaus S, Blessing F, et al. Genotyping of familial Mediterranean fever gene (MEFV)-Single nucleotide polymorphism-Comparison of Nanopore with conventional Sanger sequencing. PLoS One. 2022;17(3):e0265622. Published 2022 Mar 17.
- Watson CM, Crinnion LA, Hewitt S, et al. Cas9-based enrichment and single-molecule sequencing for precise characterization of genomic duplications. Lab Invest. 2020;100(1):135-146.
- Watson CM, Crinnion LA, Simmonds J, Camm N, Adlard J, Bonthron DT. Long-read nanopore sequencing enables accurate confirmation of a recurrent PMS2 insertion-deletion variant located in a region of complex genomic architecture. Cancer Genet. 2021;256-257:122-126.
- Valentin N, Camilleri M, Carlson P, et al. Potential mechanisms of effects of serum-derived bovine immunoglobulin/protein isolate therapy in patients with diarrhea-predominant irritable bowel syndrome. Physiol Rep. 2017;5(5):e13170.
- Kousathanas A, Pairo-Castineira E, Rawlik K, et al. Whole-genome sequencing reveals host factors underlying critical COVID-19. Nature. 2022;607(7917):97-103.
- Ivashchenko, V., de Groot, M., Derks, R. et al. Genome-wide methylation detection and episignature analysis using PacBio long-read sequencing. Genome Med 18, 11 (2026).
For research use only. Not for use in diagnostic procedures.
