Alpha Assays
Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to ELISAs.
Such targets include biomarkers, cytokines, phospho-proteins, receptor-ligand interactions, biochemical enzymes, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars, and small molecules.
Choose the homogeneous Alpha assay right for you:
AlphaLISA™ – AlphaLISA products are designed for the detection of molecules of interest in serum, plasma, cell culture supernatants, or cell lysates in a sensitive, quantitative, reproducible, and user-friendly assay. Choose from a large portfolio of ready-to-use kits or utilize the Alpha toolbox portfolio to create your own assay.
AlphaLISA™ SureFire® Ultra™ – Protein phosphorylation is a dynamic process, meticulously regulated by kinase and phosphatase cascades. This regulation is crucial for many cellular processes and has been identified as a key mechanism underlying various diseases. AlphaLISA SureFire Ultra assays provide a robust and reliable method for quantifying a targeted phosphorylation event in cell-based experiments and are less time consuming than Western Blotting.
Learn more about our focused target area and therapeutic area.
The benefits of Alpha technology
- Ease-of-use: homogeneous assay with no wash requirement and no separation steps
- Fast: results in 3 hours for most applications
- High sensitivity: detect down to femtogram levels of target
- Large dynamic range: 4-5 logs
- Compatible with many sample types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more
For research use only. Not for use in diagnostic procedures.
Explore our solutions
Target area
Explore cell-based and biochemical assays by target area of interest, including cell and gene therapy, GPCRs, targeted protein degradation, and more.
Explore cell-based and biochemical assays by target area of interest, including cell and gene therapy, GPCRs, targeted protein degradation, and more.
- Biologics screening and characterization: Explore assays for biologics screening, mechanism-of-action studies, and biomanufacturing.
- Biomarker detection: Accurate detection and quantitation of molecular targets across serum, plasma, culture supernatants, cellular lysates, and other sample types.
- Cell and gene therapy: Discover and advance cell and gene therapy research with bioanalytical immunoassays for CGT development.
- Cell signaling: Focus on cell signaling and intracellular phosphorylated proteins, with streamlined approaches to pathway analysis.
- Cytokines: Access a comprehensive range of cytokine assay kits, including interleukins, interferons, chemokines, tumor necrosis factors, and lymphokines.
- Epigenetics: Leverage our epigenetics toolbox, designed to detect specific methylation and acetylation marks on histone peptide substrates.
- GPCRs: Measure cAMP levels and track downstream protein phosphorylation with functional GPCR assays.
- Protein kinases: Explore an extensive portfolio of protein phosphorylation assays, available in cell-based or biochemical no-wash formats.
- Protein-protein interactions: Utilize highly sensitive assays to study protein interactions involved in a range of biological processes.
- Targeted protein degradation: Monitor protein expression levels and characterize the mechanism of action using our total and phospho kits.
Therapeutic area
Explore cell-based and biochemical assays by your therapeutic area of research, including oncology, neuroscience, metabolic diseases, and more.
Explore cell-based and biochemical assays by your therapeutic area of research, including oncology, neuroscience, metabolic diseases, and more.
- Autoimmune diseases: Comprehensive reagents for autoimmune disease research, including rheumatoid arthritis, lupus, inflammatory bowel disease, multiple sclerosis, and more.
- Obesity, diabetes, and metabolic diseases: Solutions for drug discovery in obesity, diabetes, and metabolic diseases, featuring assays for biomarker detection, protein analysis, and GPCR research.
- Fibrosis: Ready-to-use assays for monitoring biomarkers, phosphoproteins, and transcription factors relevant to fibrotic disease research.
- Immuno-oncology: Assays for studying immunity and immune cells, covering protein, biomarker, and cytokine detection, as well as cell toxicity and proliferation.
- Oncology: A comprehensive oncology portfolio, including assays for cytokines, biomarkers, phospho-proteins, cell proliferation, cytotoxicity, and more.
- Neuroscience: A wide range of neuroscience assays to support research in neurodegenerative disorders such as Alzheimer’s, Parkinson’s, and Huntington's disease.
- Virology: Assay technologies for viral studies, enabling assessment of viral life cycles and antiviral immune responses with streamlined workflows.
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