Improving the efficiency of metagenomic analysis
Revvity’s streamlined, automated workflow from DNA extraction to sequence ready libraries for 16S and shotgun sequencing resulting in robust data for species identification and functional profiling.
16S sequencing specifically targets and amplifies the 16S rRNA gene, a highly conserved region in microbial genomes. This method provides information about the taxonomic composition of the microbial community, allowing researchers to identify and classify different species present. It is particularly useful for studying the diversity and abundance of bacteria and archaea. 16S sequencing is cost-effective, provides phylogenetic insights, and is preferred when the focus is on understanding the overall structure and composition of the microbial community.
On the other hand, shotgun sequencing involves sequencing all the genetic material present in a sample, including the genomes of both microorganisms and the host organism(s). This method provides a more comprehensive view of the microbial community, allowing for the analysis of all genes and potential functional capabilities. Shotgun sequencing is preferred when studying complex microbial ecosystems, as it enables the identification of specific genes, functional pathways, and potential interactions within the community. It is particularly useful when the aim is to gain insights into the functional potential and metabolic activities of the microorganisms.
Improve the efficiency of your 16S and shotgun sequencing workflows with Revvity’s complete, single source workflows.
For research use only. Not for use in diagnostic procedures.