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Application Note
Automated homogenization and DNA extraction from murine GI tract tissue, cultures, and mock community for 16S sequencing
The success of sensitive downstream genomic applications such as qPCR depend on robust and reliable upstream sample preparation, which often includes proper sample/tissue homogenization and nucleic acid extraction. As the lab scales their sample throughput and their need for consistency in complex workflows, many have integrated automation-enabled protocols into their workflows. In this application note, a semi-automated workflow for nucleic acid extraction from liver tissue is presented, which leverages the Omni Bead Ruptor Elite bead mill homogenizer with the chemagic 360 nucleic acid extractor, with optional use of a JANUS automated liquid handling workstation.
For research use only. Not for use in diagnostic procedures.
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Automated homogenization and DNA extraction from murine GI tract tissue, cultures, and mock community for 16S sequencing