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NEXTFLEX Rapid XP V2 DNA-Seq Kit

The NEXTFLEX® Rapid XP V2 DNA-seq kit offers a simplified DNA-seq library preparation workflow for sequencing on Illumina® and Element Biosciences® platforms. Libraries produced using this kit are normalized during library construction using proprietary NEXTFLEX® normalization beads, delivering consistent mass and sequencing cluster density for all samples in a library pool.

For research use only. Not for use in diagnostic procedures.

For a limited time, get 20% off list price for selected NEXTFLEX library prep kits.

Promo Code: NEX20Q3
Product Variants
Part number: NOVA-5149-21
Unit Size: 8 rxns
List price: USD 288.47
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USD 288.47 /each
Part number: NOVA-5149-22
Unit Size: 48 rxns
List price: USD 1,679.00
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Part number: NOVA-5149-23
Unit Size: 96 rxns
List price: USD 3,273.00
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USD 3,273.00 /each
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Revvity
Part number: NOVA-514150
List price: USD 2,800.00
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USD 2,800.00 /each
Revvity
Part number: NOVA-534100
List price: USD 20,250.00
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Overview

  • Fully automated normalization & fragmentation
  • Enzymatic fragmentation with end-repair and A-tailing in one reaction
  • Highly efficient ligation with low adapter dimer formation
  • Efficient: Automated on the Sciclone®  and Zephyr®  NGS workstations
  • Up to 1,536 UDI Barcodes available for multiplexing

The NEXTFLEX® Rapid XP V2 DNA-seq kit includes proprietary NEXTFLEX® normalization beads, which provide a consistent mass and sequencing cluster density for all samples in a library pool. This shortens the time needed for quantification and pooling preparation for sequencing in a high throughput lab by up to 3 hours per 96 samples.

Additional product information

nextflex rapid xp v2 dna seq kit workflow
Highly Efficient Ligation with Low Adapter Formation

The NEXTFLEX  Rapid XP V2 DNA-seq kit offers a streamlined workflow with highly efficient ligation, generating low amount of adapter dimers even at low input concentrations.

nextflex rapid xp v2 dna seq kit fig1

Figure 1. Low dimer formation with NEXTFLEX Rapid XP V2 DNA-Seq kit. Different library replicates were prepared using either 500 pg (A) or 1 ng (B) of high-quality genomic DNA as input. Adapter dimer should appear as a peak at ~150 bp.

nextflex rapid xp v2 dna seq kit fig2

Figure 2. Analysis of GC bias from libraries obtained with NEXTFLEX Rapid XP V2 DNA-Seq kit. Results show minimal variability in normalized coverage across 20-60% GC content encompassing ~95% of the human genome (Right: red bars). These are consistent across 1-750 ng of genomic DNA input. Simplify Library Construction with NEXTFLEX Normalization Beads.

nextflex rapid xp v2 dna seq kit fig3
Flexible multiplexing options with 1,536 barcodes available for high-throughput multiplexing

Barcodes are not supplied with the NEXTFLEX Rapid XP DNA-seq kit v2 and need to be purchased separately. We offer 384 color-balanced NEXTFLEX UDI adapters that improve multiplexing capabilities for both low-level and high-level multiplexing needs and a set of 1,536 NEXTFLEX UDI adapters for ultra high-throughput applications.

Automation Compatibility

The NEXTFLEX Rapid XP V2 DNA-Seq kit is compatible with multiple liquid handlers, including the Sciclone G3 NGSx workstation, Sciclone G3 NGSx iQ™ workstation, and Zephyr NGS workstation.

Specifications

Brand
NEXTFLEX
Shipping Conditions
Dual Temperature
Unit Size
96 rxns

Resources

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Application Note
Low input PCR-free DNA-seq library prep and normalization

With a turn-around time of only 2.5 hours, the NEXTFLEX® Rapid XP DNA-Seq Kit v2 is a convenient workflow that can be used to...

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Flyer
Rapid XP V2 DNA-Seq Kit Flyer

The new NEXTFLEX® Rapid XP V2 DNA-seq kit includes proprietary NEXTFLEX® normalization beads*, which provide a consistent mass and...

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SDS, COAs, Manuals and more

Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.

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Adapters (8NT index)

Revvity
Part number: NOVA-514150
List price: USD 2,800.00
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Revvity
Part number: NOVA-514150-EVAL16
List price: USD 160.00
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Part number: NOVA-514150-EVAL48
List price: USD 480.00
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Revvity
Part number: NOVA-514151
List price: USD 1,920.00
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Part number: NOVA-514152
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Part number: NOVA-514153
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Targeted Sequencing

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Part number: NOVA-5143231
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Part number: NOVA-5143232
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Part number: NOVA-5143233
List price: USD 366.10
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Adapters (10NT index)

Revvity
Part number: NOVA-534100
List price: USD 20,250.00
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Revvity
Part number: NOVA-534101
List price: USD 5,063.00
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Revvity
Part number: NOVA-534102
List price: USD 5,063.00
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Revvity
Part number: NOVA-534103
List price: USD 5,063.00
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Revvity
Part number: NOVA-534104
List price: USD 11,020.00
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