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NEXTFLEX Rapid XP V2 DNA-Seq Kit
The NEXTFLEX® Rapid XP V2 DNA-seq kit offers a simplified DNA-seq library preparation workflow for sequencing on Illumina® platforms. Libraries produced using this kit are normalized during library construction using proprietary NEXTFLEX® normalization beads, delivering consistent mass and sequencing cluster density for all samples in a library pool.
For research use only. Not for use in diagnostic procedures.
Product Variants
Product information
Overview
- Fully automated normalization & fragmentation
- Enzymatic fragmentation with end-repair and A-tailing in one reaction
- Highly efficient ligation with low adapter dimer formation
- Efficient: Automated on the Sciclone® and Zephyr® NGS workstations for mid-high throughput automation and now on the BioQule™ NGS System for low throughput labs
- Up to 1,536 UDI Barcodes available for multiplexing
The NEXTFLEX® Rapid XP V2 DNA-seq kit includes proprietary NEXTFLEX® normalization beads, which provide a consistent mass and sequencing cluster density for all samples in a library pool. This shortens the time needed for quantification and pooling preparation for sequencing in a high throughput lab by up to 3 hours per 96 samples.
Additional product information
Highly Efficient Ligation with Low Adapter Formation
The NEXTFLEX Rapid XP V2 DNA-seq kit offers a streamlined workflow with highly efficient ligation, generating low amount of adapter dimers even at low input concentrations.
Figure 1. Low dimer formation with NEXTFLEX Rapid XP V2 DNA-Seq kit. Different library replicates were prepared using either 500 pg (A) or 1 ng (B) of high-quality genomic DNA as input. Adapter dimer should appear as a peak at ~150 bp.
Figure 2. Analysis of GC bias from libraries obtained with NEXTFLEX Rapid XP V2 DNA-Seq kit. Results show minimal variability in normalized coverage across 20-60% GC content encompassing ~95% of the human genome (Right: red bars). These are consistent across 1-750 ng of genomic DNA input. Simplify Library Construction with NEXTFLEX Normalization Beads.
Flexible multiplexing options with 1,536 barcodes available for high-throughput multiplexing
Barcodes are not supplied with the NEXTFLEX Rapid XP DNA-seq kit v2 and need to be purchased separately. We offer 384 color-balanced NEXTFLEX UDI adapters that improve multiplexing capabilities for both low-level and high-level multiplexing needs and a set of 1,536 NEXTFLEX UDI adapters for ultra high-throughput applications.
Automation Compatibility
The NEXTFLEX Rapid XP V2 DNA-Seq kit is compatible with multiple liquid handlers, including the Sciclone G3 NGSx workstation, Sciclone G3 NGSx iQ™ workstation, and Zephyr NGS workstation. For low throughput automation, the NEXTFLEX Rapid XP V2 DNA-Seq kit is also now automated on the BioQule™ NGS System which simplifies both library prep AND quantitation. This low-cost, benchtop instrument delivers libraries ready to load into your sequencer with only 15 minutes of hands-on time.
Specifications
| Brand |
NEXTFLEX
|
|---|---|
| Unit Size |
8 rxns
|
Resources
Application Note
Low input PCR-free DNA-seq library prep and normalization
With a turn-around time of only 2.5 hours, the NEXTFLEX® Rapid XP DNA-Seq Kit v2 is a convenient workflow that can be used to...
Flyer
Rapid XP V2 DNA-Seq Kit Flyer
The new NEXTFLEX® Rapid XP V2 DNA-seq kit includes proprietary NEXTFLEX® normalization beads*, which provide a consistent mass and...
SDS, COAs, Manuals and more
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