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SureLight Allophycocyanin-anti-Mouse Antibody, 1 mg

APC-labeled anti-mouse IgG antibody, for capturing mouse IgG antibodies in LANCE TR-FRET assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AD0059M
Part Number: AD0059M
  • Goat polyclonal antibody against mouse IgG conjugated to allophycocyanin (APC). APC is a fluorescent light harvesting protein unique to cyanobacteria and red algae and a member of the phycobiliprotein family of direct fluorescent dyes. Surelight APC is a chemically and intramolecularly cross-linked form of a protein. It is produced using a proprietary manufacturing process to increase thermal stability while maintaining the fluorescent and spectral properties of native dye.

    LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

  • Antibody Conjugates
    Anti-mouse IgG
    Application
    Protein Analysis & Detection
    Automation Compatible
    Yes
    Brand
    LANCE SureLight
    Detection Method
    Time-Resolved Fluorescence (TRF)
    TR-FRET
    Experimental Type
    In vitro
    Fluorophore
    SureLight APC
    Format
    Microplates

Resources

1-1 of 1 Resources
Application Note Icon Image
Application Note
Developing a LANCE TR-FRET assay for screening PD-1 and PD-L1 binding inhibitors.
lance, tr-fret, pd1, pd-1, pdl1, inhibitors, assays