Skip to main content

LANCE Ultra Phospho-STAT3 (Tyr705 ) Detection Kit, 500 Assay Points

LANCE Ultra phospho-STAT3 (Tyr705) kits are designed for the detection of phosphorylated STAT3 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: TRF4004C
Unit Size: 500 Assay Points
Part Number: TRF4004M
Unit Size: 10,000 Assay Points
  • Please note control lysates are NOT included in the kits. Control lysates are sold separately, catalog number TRF4004S.

    Formats:

    • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
    • Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

    LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

    STAT3 is an important transcriptional activator, and is involved in regulating cytokines and growth factor receptors. STAT3 is phosphorylated at Tyr-705 by the JAK pathway, upon which STAT3 will dimerize, translocate to the nucleus, and bind DNA. In some cancers STAT3 is shown to be constitutively activated. It has been implicated in both anti-apoptotic and oncogenic pathways.

  • Application
    Kinase
    Protein Analysis & Detection
    Assay Pathway
    JAK/STAT
    Assay Points
    5000
    Assay Target Class
    Phospho-protein
    Automation Compatible
    Yes
    Brand
    LANCE Ultra
    Detection Method
    Time-Resolved Fluorescence (TRF)
    TR-FRET
    Shipping Conditions
    Shipped in Blue Ice
    Therapeutic Area
    Autoimmunity
    Neuroscience
    Respiratory
    Unit Size
    500 Assay Points

Resources

1-1 of 1 Resources
Application Note Icon Image
Application Note
LANCE Ultra TR-FRET-based detection and modulation of phosphorylated STAT3 levels in human cells.

STAT3 is a particularly important therapeutic target since it is constitutively active in a range of cancer cells lines and human...