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LANCE Ultra Total STAT3 Detection Kit, 10,000 Assay Points

LANCE Ultra total STAT3 kits are designed for the detection of total STAT3 (phosphorylated and unphosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-STAT3, and is compatible with both adherent and suspension cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: TRF4005M
Unit Size: 10,000 Assay Points
Part Number: TRF4005C
Unit Size: 500 Assay Points
  • Please note control lysates are NOT included in kits. Control lysates are sold separately, catalog number TRF4004S.

    Formats:

    • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
    • Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

    LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

    STAT3 is an important transcriptional activator, and is involved in regulating cytokines and growth factor receptors. STAT3 is phosphorylated at Tyr-705 by the JAK pathway, upon which STAT3 will dimerize, translocate to the nucleus, and bind DNA. In some cancers STAT3 is shown to be constitutively activated. It has been implicated in both anti-apoptotic and oncogenic pathways.

  • Application
    Kinase
    Protein Analysis & Detection
    Assay Pathway
    JAK/STAT
    Assay Target
    STAT3
    Assay Target Class
    Protein
    Automation Compatible
    Yes
    Brand
    LANCE Ultra
    Detection Method
    Time-Resolved Fluorescence (TRF)
    TR-FRET
    Therapeutic Area
    Autoimmunity
    Neuroscience
    Respiratory
    Unit Size
    10,000 Assay Points

Resources

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Application Note Icon Image
Application Note
LANCE Ultra TR-FRET-based detection and modulation of phosphorylated STAT3 levels in human cells.
LANCE, STAT3, TR-FRET, STAT, assays, target