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LANCE Ultra Total SLP76 Detection Kit, 500 Assay Points

LANCE Ultra total SLP-76 kits are designed for the detection of SLP-76 (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalizaton assay for phospho-SLP-76, and is compatible with both adherent and suspension cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: TRF4017C
Unit Size: 500 Assay Points
List price: USD 2,043.00
Your price:
USD 2,043.00
USD 2,043.00 /each
Part number: TRF4017M
Unit Size: 10,000 Assay Points
List price: USD 9,232.00
Your price:
USD 0.00
USD 9,232.00 /each

Overview

Please note control lysates are NOT included in kits. Control lysates are sold separately, catalog number TRF4010S.

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

Specifications

Application
Kinase
Protein Analysis & Detection
Assay Points
500
Assay Target Class
Phospho-protein
Automation Compatible
Yes
Brand
LANCE Ultra
Detection Method
Time-Resolved Fluorescence (TRF)
TR-FRET
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Autoimmunity
Immuno-oncology
Inflammation
Neuroscience
Oncology
Unit Size
500 Assay Points

Resources

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Application Note
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