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LANCE Ultra Phospho-NFkB (Ser536) Detection Kit, 10,000 Assay Points

LANCE Ultra phospho-NFκB (Ser536) kits are designed for the detection of phosphorylated NFκB in cell lysates using a simple, homogeneous LANCE Ultra TR-FRET assay (no wash steps). This assay is compatible with both adherent and suspension cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: TRF4019M
Unit Size: 10,000 Assay Points
Part Number: TRF4019C
Unit Size: 500 Assay Points
  • Please note control lysates are sold separately, catalog number TRF4019S.

    Formats:

    • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
    • Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

    LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

    NFκB (Nuclear Factor kappa B) is a superfamily comprised of p65, RelB, c-Rel, p50/p105, and p52/p100. The combinations of these as either homo- or heterodimers help to regulate the immune system in response to infection or cellular stress. The classical NFκB pathway involves p65 and p50 activation and in stimulation of cytokines or activation of TLR. Phosphorylation at S536 has been shown to enhance transcriptional activity in the nucleus of cytokines as part of immune response. Several auto-immune disorders have been traced back to the deregulation of the NFκB pathway.

  • Automation Compatible
    Yes
    Application
    Kinase
    Protein Analysis & Detection
    Assay Points
    10000
    Assay Target
    NFκB
    Assay Target Class
    Phospho-protein
    Detection Method
    Time-Resolved Fluorescence (TRF)
    TR-FRET
    Therapeutic Area
    Metabolic
    Assay Pathway
    NFκB
    Brand
    LANCE Ultra
    Unit Size
    10,000 Assay Points

Resources

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Application Note Icon Image
Application Note
LANCE Ultra TR-FRET-based detection and modulation of phosphorylated STAT3 levels in human cells.
LANCE, STAT3, TR-FRET, STAT, assays, target