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LANCE Ultra Phospho-LCK (Tyr394) Detection Kit, 500 Assay Points

LANCE Ultra phospho-LCK (Tyr394) kits are designed for the detection of phosphorylated LCK in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: TRF4026C
Unit Size: 500 Assay Points
List price: USD 2,043.00
Your price:
USD 2,043.00
USD 2,043.00 /each
Part number: TRF4026M
Unit Size: 10,000 Assay Points
List price: USD 9,232.00
Your price:
USD 0.00
USD 9,232.00 /each


Please note control lysates are sold separately, catalog number TRF4026S.


  • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

LCK is a cytoplasmic phosphoprotein from the Src family of non-receptor tyrosine kinases. LCK regulates T cell activation by associating with CD4 and CD8 surface receptors on T cells. Phosphorylation at Y394 enhances kinase activity. LCK is associated with Immunodeficiency 22 and CD45 deficiency. No-death receptor apoptosis is also involves LCK.


Protein Analysis & Detection
Assay Points
Assay Target Class
Automation Compatible
Detection Method
Time-Resolved Fluorescence (TRF)
Shipping Conditions
Shipped in Blue Ice
Unit Size
500 Assay Points


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Application Note
LANCE Ultra TR-FRET-based detection and modulation of phosphorylated STAT3 levels in human cells.

STAT3 is a particularly important therapeutic target since it is constitutively active in a range of cancer cells lines and human...

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