Skip to main content

LANCE Ultra Phospho-ERK (Thr202/Tyr204) Detection Kit, 500 Assay Points

LANCE Ultra phospho-ERK 1/2 (Thr202/Tyr204) kits are designed for the detection of phosphorylated ERK 1 and ERK2 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: TRF4000C
Unit Size: 500 Assay Points
Part Number: TRF4000M
Unit Size: 10,000 Assay Points
  • Please note control lysates are NOT included in the kits. Control lysates are sold separately, catalog number TRF4000S.

    Formats:

    • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
    • Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

    LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

    ERK1 and ERK2, also known as MAP Kinase 3 and MAP Kinase 1, are serine/threonine kinases and important targets for cancer treatment. ERK kinases are dually phosphorylated at Tyr-202 and Tyr-204, leading to activation of the target. ERK can also be dephosphorylated at Tyr-202 by PTPRJ. A number of downstream targets have been identified including EIF4EBP1, which is necessary for the initiation of translation. It has also been shown to phosphorylate ELK-1.

  • Application
    Kinase
    Protein Analysis & Detection
    Assay Pathway
    MAPK
    Assay Points
    5000
    Assay Target Class
    Phospho-protein
    Automation Compatible
    Yes
    Brand
    LANCE Ultra
    Detection Method
    Time-Resolved Fluorescence (TRF)
    TR-FRET
    Shipping Conditions
    Shipped in Blue Ice
    Therapeutic Area
    Cardiovascular
    Metabolic
    Neuroscience
    Unit Size
    500 Assay Points

Resources

1-1 of 1 Resources
Application Note Icon Image
Application Note
LANCE Ultra TR-FRET-based detection and modulation of phosphorylated STAT3 levels in human cells.

STAT3 is a particularly important therapeutic target since it is constitutively active in a range of cancer cells lines and human...