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LANCE Ultra Phospho-ERK (Thr202/Tyr204) Detection Kit, 500 Assay Points

LANCE Ultra phospho-ERK 1/2 (Thr202/Tyr204) kits are designed for the detection of phosphorylated ERK 1 and ERK2 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: TRF4000C
Unit Size: 500 Assay Points
List price: USD 2,043.00
Your price:
USD 2,043.00
USD 2,043.00 /each
Part number: TRF4000M
Unit Size: 10,000 Assay Points
List price: USD 9,232.00
Your price:
USD 0.00
USD 9,232.00 /each


Please note control lysates are NOT included in the kits. Control lysates are sold separately, catalog number TRF4000S.


  • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

ERK1 and ERK2, also known as MAP Kinase 3 and MAP Kinase 1, are serine/threonine kinases and important targets for cancer treatment. ERK kinases are dually phosphorylated at Tyr-202 and Tyr-204, leading to activation of the target. ERK can also be dephosphorylated at Tyr-202 by PTPRJ. A number of downstream targets have been identified including EIF4EBP1, which is necessary for the initiation of translation. It has also been shown to phosphorylate ELK-1.


Protein Analysis & Detection
Assay Pathway
Assay Points
Assay Target Class
Automation Compatible
Detection Method
Time-Resolved Fluorescence (TRF)
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Unit Size
500 Assay Points


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