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LANCE Eu-W1024 Anti-PY20 Antibody, 1mg

Europium-labeled anti-phosphotyrosine (PY20) antibody, for use in LANCE® TR-FRET kinase assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AD0066
Unit Size: 50 µg
Part Number: AD0067
Unit Size: 1 mg
  • The PY20 antibody (IgG2b) and PT66 antibody are mouse monoclonal antibodies that bind to phosphorylated tyrosine residues. Eu-W1024 labeled anti-phosphotyrosine PY20 antibody for LANCE® assays where the analyte concentration is < 1-10 nM and assay pH > 7. The PY20 antibody is a mouse monoclonal antibody, IgG2b, that binds to phosphorylated tyrosine residues.

    Generic LANCE® reagents are intended for setting up homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) based assays using Eu-Chelate label as a donor and APC-labeled reagent as an acceptor. Generic reagents facilitate setting up LANCE® assays when there is a limited amount of specific reagents available or the assay reagents are relatively unstable. To enable optimization of both the signal or the noise ratio and signal stability, some of the generic reagents are labeled with two different chelates, W1024 or W8044.

    LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

  • Antibody Conjugates
    PY20
    Application
    Kinase
    Automation Compatible
    Yes
    Brand
    LANCE
    Detection Method
    Time-Resolved Fluorescence (TRF)
    TR-FRET
    Experimental Type
    In vitro
    Fluorophore
    Eu-W1024
    Format
    Microplates
    Molecular Modification
    Phosphorylation
    Shipping Conditions
    Shipped in Dry Ice
    Unit Size
    1 mg

Resources

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Application Note
Measurement of p38/MAPK Activity Using LANCE

This note presents a method to evaluate inhibitors of p38 kinase activity using LANCE. We are measuring energy transfer from a Eu...