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LANCE Eu-W1024 Anti-Mouse IgG Antibody, 1mg

Europium-labeled anti-mouse IgG, for capturing antibodies in LANCE® TR-FRET assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AD0076
Unit Size: 50 µg
Part Number: AD0077
Unit Size: 1 mg
  • Eu-W1024 labeled anti-mouse IgG antibody for LANCE® assays where analyte concentration < 1-10 nmol/L and assay pH > 7. The universal reagent for detection of mouse antibodies consists of the IgG fraction of anti-mouse antibodies raised in rabbit. There are from 6 to 10 Eu3+ ions per IgG. Interference from human, ox and rat antibodies can usually be removed by adding serum from the other species to the assay buffer.

    Generic LANCE® reagents are intended for setting up homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) based assays using Eu-Chelate label as a donor and APC-labeled reagent as an acceptor. Generic reagents facilitate setting up LANCE® assays when there is a limited amount of specific reagents available or the assay reagents are relatively unstable. To enable optimization of both the signal or the noise ratio and signal stability, some of the generic reagents are labeled with two different chelates, W1024 or W8044.

    LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

  • Antibody Conjugates
    Anti-mouse IgG
    Application
    Protein Analysis & Detection
    Automation Compatible
    Yes
    Brand
    LANCE
    Detection Method
    Time-Resolved Fluorescence (TRF)
    TR-FRET
    Experimental Type
    In vitro
    Fluorophore
    Eu-W1024
    Format
    Microplates
    Unit Size
    1 mg

Resources

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Application Note
Developing a LANCE TR-FRET assay for screening PD-1 and PD-L1 binding inhibitors.

Here, we demonstrate the utility and benefits of using LANCE Ultra TR-FRET assay technology for identifying and characterizing PD...