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LANCE Ultra Anti-Phospho-MEK1/2 (Ser217/221) Antibody, 1,562 Assay Points

Rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human MEK1 phosphorylated at Ser217 and Ser221. It also reacts with MEK1 singly phosphorylated at Ser217 or Ser221. MEK1 is phosphorylated in vivo by RAF1 and COT. Homologous phosphorylation sites are found in MEK2. Sequence motif recognized: GQLIDSMANSFVG

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: TRF0213-D
Unit Size: 10 µg
List price: USD 3,110.00
Your price:
USD 3,110.00
USD 3,110.00 /each
Part number: TRF0213-M
Unit Size: 100 µg
List price: USD 9,966.00
Your price:
USD 0.00
USD 9,966.00 /each

Overview

LANCE Ultra time-resolved fluorescence resonance energy transfer (TR-FRET) assays use a proprietary europium chelate donor dye, W1024 (Eu), together with ULight™, a small molecular weight acceptor dye with a red-shifted fluorescent emission. Validated for over 310 kinases, we provide data transparency by supplying:

  • Test conditions: Rapid assay setup to move straight into optimized assay performance
  • S:B data: Confidence in proven performance of the kinase on specific substrates; no guessing
  • Phospho-motifs: Substrate sequences are provided
  • Assay options: Multiple substrates are tested so you can select the best peptide sequence and S:B for your kinase

For this antibody:

  • 10 μg = 1,562 assay points
  • 100 μg = 15,625 assay points

In this kinase assay configuration, the phosphorylated biotinylated substrate is detected by both the ULight-labeled streptavidin and Eu-labeled anti-phosphosubstrate antibodies, which brings donor and acceptor dyes into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight substrate phosphorylation.

Specifications

Antibody Conjugates
Anti-phospho-MEK1/2
Application
Kinase
Automation Compatible
Yes
Brand
LANCE Ultra
Detection Method
Time-Resolved Fluorescence (TRF)
TR-FRET
Experimental Type
In vitro
Fluorophore
Eu-W1024
Format
Microplates
Molecular Modification
Phosphorylation
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Autoimmunity
Immuno-oncology
Metabolic
Neuroscience
Oncology
Unit Size
10 µg

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