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LANCE Ultra Anti-acetyl K9 Histone H3 Antibody, 10µg

Europium-labeled monoclonal antibody recognizing human Histone H3 acetylated at lysine 9 (H3K9ac). For use in LANCE® Ultra epigenetic writer and eraser assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Part number: TRF0400-D
Unit Size: 10 µg
List price: USD 4,966.00
Your price:
USD 4,966.00
USD 4,966.00 /each
Part number: TRF0400-M
Unit Size: 100 µg
List price: USD 12,490.00
Your price:
USD 0.00
USD 12,490.00 /each

Overview

The LANCE® Ultra Europium-anti-acetyl-Histone H3 Lysine 9 (H3K9ac) antibody was used for the development and optimization of a p300 acetyltransferase assay using a biotinylated Histone H3 (1-21) peptide as substrate. A technical note describing the assay is available in our product literature.

Formats:

  • 10 µg = 1,500 assay points (384-well assay format)
  • 100 µg = 15,000 assay points (384-well format)

Proven, cost-efficient LANCE Ultra reagents can be used to quantitate peptide modifications, detecting specific meythylation and acetylation states. No wash, homogenous LANCE Ultra reagents are HTS friendly - design your own epigenetics screening strategy for greatest efficiency.

Epigenetic enzymatic assays are optimized using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody (Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecules into close proximity. Upon irradiation at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of biotinylated substrate modification.

Specifications

Antibody Conjugates
Anti-H3K9ac
Application
Epigenetics
Automation Compatible
Yes
Brand
LANCE Ultra
Detection Method
Time-Resolved Fluorescence (TRF)
TR-FRET
Experimental Type
In vitro
Fluorophore
Eu-W1024
Format
Microplates
Molecular Modification
Acetylation
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Oncology
Unit Size
10 µg

Resources

1-1 of 1 Resources
Whitepaper
Epigenetic modifications and how they relate to cancer development and therapy

Epigenetic modifications in cancer

The field of epigenetics has revealed revolutionary methods for understanding heredity...

SDS, COAs, Manuals and more Illuminator

SDS, COAs, Manuals and more

Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.

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