Skip to main content

HTRF

Reagent-htrf.jpg

HTRF® (Homogeneous Time Resolved Fluorescence) is a no-wash technology that combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence.

This technology can be utilized in cellular or biochemical assays, employing competitive and non-competitive formats. It may be used  for drug discovery, biotherapy and basic research in therapeutic areas such as oncology, CNS and metabolic and immune disorders. Researchers can explore a wide range of applications, including GPCRs, kinases, epigenetics, biotherapy, and quantification of a range of biomarkers and cytokines.

  • Easy to perform: homogeneous assay with no wash requirement
  • High level of sensitivity, signal stable in time
  • Time and labor savings, rapid and accurate results
  • Amenable to miniaturization
  • Ability to detect and analyze small and large complexes with low to high affinity interactions
  • Field of applications: basic research, target discovery and validation, HTS and primary screening, secondary screening, lead optimization 
Image-1_cell-signaling_HTRF-category-page_252x252
Cell signaling

The phosphorylation reaction is a post-translational modification, mediated by kinases that controls the activity of proteins involved in signal transduction. It is an appropriate readout of the activation status of a pathway of interest.  

The phosphorylation reaction is a post-translational modification, mediated by kinases that controls the activity of proteins involved in signal transduction. It is an appropriate readout of the activation status of a pathway of interest.  

The HTRF cell signaling kits are designed to  directly measure proteins within cells. We offer more than 200 ready-to-use HTRF kits for detecting endogeneous and basal levels of phosphorylated proteins. All kits are built on the same precise format and protocol.

  • Cell-based assays, measure your phospho protein of interest
  • No-wash, no radioactivity, no gel
  • 16 μL sample
  • Equal or more sensitive than Western Blot
  • Pharmacological studies of pathway inhibitors, agonists, and allosteric modulators
  • Compatible with cell lysates, tissue extracts, xenografts, iPS cells, 3-D cell models

Learn more

Image-2_GPCR-research_HTRF-category-page_252x252
GPCR research

Revvity is a leader in G protein-coupled receptor research and drug discovery, with over 100 products and compelling scientific content in many categories of GPCR Binding and signaling. Each year, hundreds of GPCR researchers around the globe trust our products to go further and faster in their research projects.

Revvity is a leader in G protein-coupled receptor research and drug discovery, with over 100 products and compelling scientific content in many categories of GPCR Binding and signaling. Each year, hundreds of GPCR researchers around the globe trust our products to go further and faster in their research projects.

  • Gs-, Gi/o-, Gq-coupled receptor activation
  • Agonist, antagonist, inverse agonist, and allosteric modulator screening
  • Detection of slow acting agonists and antagonists
  • Phosphodiesterase (PDE) monitoring
  • Adenylate cyclase activity assessment 

Learn more

Image-3_Biomarkers_HTRF-category-page_252x252
Biomarkers

HTRF kits are developed to measure released and circulating biomarkers. This offering covers multiple research areas, such as CNS, cardiovascular, inflammation, oncology, pain, metabolism, NASH, and diabetes.

HTRF kits are developed to measure released and circulating biomarkers. This offering covers multiple research areas, such as CNS, cardiovascular, inflammation, oncology, pain, metabolism, NASH, and diabetes.

  • Simple add-and-read method, alternative to ELISA
  • < 1 hour bench time
  • Compatible with Revvity 96-well low volume plate
  • All components included in Kit

Learn more

Image-4_Cytokines_HTRF-category-page_252x252
Cytokines

Our HTRF kits cover the full range of cytokines: interleukins, interferons (IFN), and chemokines. All our assays enable the measurement of cytokine release in cell supernatant.

Our HTRF kits cover the full range of cytokines: interleukins, interferons (IFN), and chemokines. All our assays enable the measurement of cytokine release in cell supernatant.

Our assays have been validated in several cell lines, primary cells, and PBMCs. The low sample requirement allows the quantification of multiple cytokines in parallel, from the same supernatant.

  • 16 μL sample
  • < 2 hrs bench time
  • Validated on PBMC
  • Compatible with Revvity 96- and 384-well low-volume plates

Learn more

Image-5_Protein-protein-int_HTRF-category-page_252x252
Protein-protein interactions

We offer an extensive line of ready-to-use protein-protein interaction reagents labeled with HTRF fluorescent dyes (europium- and terbium-cryptates, XL665, and d2) for the development of novel or customer-specific assays.

We offer an extensive line of ready-to-use protein-protein interaction reagents labeled with HTRF fluorescent dyes (europium- and terbium-cryptates, XL665, and d2) for the development of novel or customer-specific assays.

A wide range of assays have already been produced with these reagents and have proven relevant when addressing interactions of widely varying sizes, affinities, and formats.

  • Biotherapeutics screening
  • Protein-protein interactions: small to large complexes, low to high affinity
  • Nuclear receptor assays
  • Protease and other enzyme assays
  • Protein-DNA interaction

Learn more

Image-6_epigenetic_HTRF-category-page_252x252
Epigenetics

We provides a way to explore epigenetic enzymes and proteins such as histone methyltransferases (HMT), demethylases (HDMT), deacetylases (HDAC), sirtuins, and Binding domains as druggable targets.

We provides a way to explore epigenetic enzymes and proteins such as histone methyltransferases (HMT), demethylases (HDMT), deacetylases (HDAC), sirtuins, and Binding domains as druggable targets.

We offer EPIgeneous® products, a dedicated line of Kits in biochemical and cellular formats for investigations of DNA methyltransferases (DNMTs), HDMTs, HMTs, HDACs, and Ubiquitin ligases.

  • Detecting peptide, nucleosome, full histone, DNA, RNA, and small molecule modifications
  • Screening and characterization of enzyme inhibitors

Learn more

Image-7_kinase_HTRF-category-page_252x252
Kinase

Benefit from our extensive HTRF-based line of biochemical and kinase assays and reagents for analyzing serine/threonine, tyrosine kinases. This platform is available in semi-universal and kinase-specific assay formats.

Benefit from our extensive HTRF-based line of biochemical and kinase assays and reagents for analyzing serine/threonine, tyrosine kinases. This platform is available in semi-universal and kinase-specific assay formats.

All HTRF biochemical kinase assays use nonradioactive, homogeneous protocols, and are compatible with a range of ATP concentrations and substrate types, including whole proteins. We developed a line of kinase binding assays to specifically monitor kinase/inhibitor interactions and complement its existing kinase activity assay solutions. The assays can be easily miniaturized for increased throughput, reduced reagent usage, and lower enzyme consumption.

  • Validated on over 200 kinases
  • Low enzyme consumption
  • Broad range of ATP (up to mM)
  • Study of inactive or mutated kinases

Learn more

bg-element-listing-page bg-element-listing-page-mobile
Products & Services
Resource Library
Filters
Expand All | Collapse All

View Product Listing Solr Page, display Block: Application

View Product Listing Solr Page, display Block: Assay Technology

Assay Technology

View Product Listing Solr Page, display Block: Brand

View Product Listing Solr Page, display Block: Detection Method

Detection Method

View Product Listing Solr Page, display Block: Item Class

Sort by: Recommended
Recommended
Product Name A-Z
Product Name Z-A
1 - 25 of 1136 Products and Services
Filter & Sort
Active Filters ()
Clear All
New
Picture of HTRF Human Total IKZF3 Control Lysate
New
Shipping box for Revvity reagent kits
USD 2,147.00 - 12,490.00
New
Picture of HTRF Human and Mouse LC3B-II Detection Kit
USD 2,147.00 - 12,490.00
New
Picture of HTRF Human Androgen Receptor Variant 7 Detection Kit
New
Picture of HTRF β-Arrestin 2 Control Lysate
New
Picture of HTRF Human Total Cyclin B1 Control Lysate
New
Picture of HTRF Human Total Cyclin B1 Detection Kit
USD 2,147.00 - 12,490.00
New
HTRF Anti-VHH-d2-5000-assay-points
USD 650.00 - 2,079.00
New
Picture of HTRF Human Total IDO1 Control Lysate
New
Picture of HTRF FLAG Check Kit
USD 1,039.00 - 6,819.00
New
Picture of HTRF Human and Mouse LC3B-II Control Lysate
New
Picture of HTRF FLAG Check Standard
New
Picture of HTRF Human Total IKZF3 Detection Kit
USD 2,147.00 - 12,490.00
New
Picture of HTRF MAb Anti FLAG-Tb
USD 262.00 - 1,911.00
New
Picture of HTRF Human Ataxin 2 Mutant Detection Kit
USD 1,372.00 - 14,590.00
New
Picture of HTRF Human IL-15 Detection Kit
USD 1,103.00 - 10,290.00
New
Picture of HTRF Human and Mouse Total IKZF1 Detection Kit
New
Picture of HTRF Human Phospho-VAV1 (Tyr174) Detection Kit
New
Picture of HTRF Human Ataxin 2 Mutant Control Lysate
New
Picture of HTRF Human Total IDO1 Detection Kit
USD 2,147.00 - 12,490.00
Filters
Expand All | Collapse All

View Resource Library Media Listing, display Block: Resource Type

Sort by: Resource Name A-Z
Resource Name A-Z
Resource Name Z-A
1 - 5 of 5 Resources
Filter & Sort
Active Filters ()
Clear All
Technical Note
Developing a high throughput AlphaLISA assay for screening activity of biologics produced by engineered probiotic microbes
Biologics Tenza AlphaLISA High Throughput Screening protein therapeutics
Learn More
Application Note Icon Image
Application Note
Optimization and pharmacological validation of wild type beta-arrestin 2 plasmid transfection.
htrf gpcr beta-arrestin 2 plasmid transfection optimization
Learn More
Flyer
Reagent solutions for autoimmunity research.
alphalisa alpha htrf surefire autoimmunity flyer
Learn More
Flyer
Reagent solutions for PROTAC research
protac htrf alpha
Learn More
Flyer
Therapeutic development with robust cellular binding assays.
htrf flyer assays therapeutic development
Learn More