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Western Lightning ONE Femto Ultra, Chemiluminescent Substrate (250 mL)

When proteins are low and antibodies are precious, our extreme-level pre-mixed one-component chemiluminescent horseradish peroxidase (HRP) substrate with low antibody consumption is the right choice. Western Lightning ONE Femto Ultra can detect low-femtogram protein levels in western blots with superior signal intensity.

For research use only. Not for use in diagnostic procedures.
Part Number: NEL150001EA
Unit Size:
Part Number: NEL151001EA
Unit Size:
Part Number: NEL152001EA
Unit Size:
  • Our most sensitive substrate in our premier line of premixed chemiluminescent HRP substrates, Western Lightning ONE Femto Ultra offers low-femtogram detection with excellent signal intensity. Western Lightning ONE Femto Ultra is designed with maximum sensitivity in a single reagent format. Stored and ready-to-use at room temperature, the 12 month extended shelf life means you can buy and use Western Lightning ONE Femto Ultra with confidence. Unit size 2 x 125 mL.

    Comparison of Western Lightning ONE Versus Major Competitors

    Performance and Signal Duration

    • Western blotting detection of HDAC-1 in HeLa cell lysate
    • Exposure time is 120 secs for each time point
    • Images captured with ImageQuant LAS 4000 from GE Healthcare
  • Application
    Western blotting
    Western Lightning ONE
    Detection Method
    Sample Type
    Chemiluminescent Substrate


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The power of Western Lightning ONE HRP chemiluminescent substrates
Western Lightning, Western lightning ONE, western blots, western blotting, HRP substrates, HRP chemiluminescent substrates, horseradish peroxidase substrates, western blotting reagents, chemiluminescent substrates
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Application Note
Western blot validation of CRISPR-Cas9-induced functional knockout using synthetic sgRNA molecules
CRISPR, CRISPR Cas9, Cas9. CRISPR-Cas9, western blot, western blotting, western lightning ONE, chemiluminscent substrates, sgRNA, CRSIPR validation, Horizon, Horizon discovery, BioLegend
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Western Blotting Solutions
Western blotting, protein detection, western lightning, chromogenic substrates, chemiluminescent substrates, transfer membranes, secondary antibodies, primary antibodies