Skip to main content

Western Lightning ONE Femto, Chemiluminescent Substrate (25 mL)

A versatile, mid-level pre-mixed, one-component chemiluminescent horseradish peroxidase (HRP) substrate appropriate for a wide range of antibody dilutions, Western Lightning ONE Femto can detect mid-femtogram protein levels in western blots with a high signal intensity.

For research use only. Not for use in diagnostic procedures.
Part Number: NEL140001EA
Unit Size:
Part Number: NEL141001EA
Unit Size:
Part Number: NEL142001EA
Unit Size:
  • Get accurate detection of both low and high abundance proteins in the same experient. Part of our premier line of premixed chemiluminescnet HRP substrates, Western Lightning ONE Femto offers the flexibility of mid-femtogram detection with excellent dynamic range. This substrate offers sensitive and consistent detection of proteins with no mixing or measuring parts required. Stored and ready-to-use at room temperature, the 12 month extended shelf life means you can buy and use Western Lightning ONE Femto with confidence. Available in 25 mL size.

    Comparison of Western Lightning ONE Versus Major Competitors

    Performance and Signal Duration

    • Western blotting detection of HDAC-1 in HeLa cell lysate
    • Exposure time is 120 secs for each time point
    • Images captured with ImageQuant LAS 4000 from GE Healthcare
  • Application
    Western blotting
    Western Lightning ONE
    Detection Method
    Sample Type
    Chemiluminescent Substrate


1-3 of 3 Resources
The power of Western Lightning ONE HRP chemiluminescent substrates
Western Lightning, Western lightning ONE, western blots, western blotting, HRP substrates, HRP chemiluminescent substrates, horseradish peroxidase substrates, western blotting reagents, chemiluminescent substrates
Application Note Icon Image
Application Note
Western blot validation of CRISPR-Cas9-induced functional knockout using synthetic sgRNA molecules
CRISPR, CRISPR Cas9, Cas9. CRISPR-Cas9, western blot, western blotting, western lightning ONE, chemiluminscent substrates, sgRNA, CRSIPR validation, Horizon, Horizon discovery, BioLegend
Brochure Icon Image
Western Blotting Solutions
Western blotting, protein detection, western lightning, chromogenic substrates, chemiluminescent substrates, transfer membranes, secondary antibodies, primary antibodies