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Western Lightning ONE Pico, Chemiluminescent Substrate (25 mL)

Our one-component chemiluminescent horseradish peroxidase (HRP) substrates are ideal for routine analysis detection of abundant proteins. Western Lightning™ ONE Pico can detect mid-picogram protein levels in western blots and provides low background for a high signal to noise ratio.

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MARCH10
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Our one-component chemiluminescent horseradish peroxidase (HRP) substrates are ideal for routine analysis detection of abundant proteins. Western Lightning™ ONE Pico can detect mid-picogram protein levels in western blots and provides low background for a high signal to noise ratio.

Click to copy promo code to clipboard.
March lab savings - buy on Revvity.com or Revvity punchout to SAVE 10% with promo code!
MARCH10
Sale ends 3/24. Terms and conditions apply.
Product Variants
Unit Size: 25 mL
Part #:
NEL130001EA
List Price
USD 76.31
Your online price:
Unit Size: 250 mL
Part #:
NEL131001EA
List Price
USD 250.34
Your online price:
Unit Size: 2 x 250 mL
Part #:
NEL132001EA
List Price
USD 414.12
Your online price:
For research use only. Not for use in diagnostic procedures.

Overview

Part of our premier line of premixed chemiluminescent HRP substrates, Western Lightning ONE Pico offers mid-picogram detection with no mixing required. Designed for sensitive and consistent detection of proteins, this single reagent format enables greater experimental consistency while remaining an economical choice for your everyday detection needs. Stored and ready-to-use at room temperature, the 12 month extended shelf life means you can buy and use Western Lightning ONE Pico with confidence. Available in 25 mL size.

Comparison of Western Lightning™ ONE Versus Major Competitors

Performance and Signal Duration

  • Western blotting detection of HDAC-1 in HeLa cell lysate
  • Exposure time is 120 secs for each time point
  • Images captured with ImageQuant™ LAS 4000 from GE Healthcare

Additional product information

Performance Signal Duration

Detection Level: Mid-picogram

wlo-pico-1
wlo-pico-web
  • Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane
  • Primary antibody:Rabbit-anti Human HDAC-1 1:2000
  • Secondary antibody:Goat anti-rabbit IgG HRP (2mg/mL) 1:20000

Detection Level: Mid-femtogram

wlo-femto-web
wlo-femto-web
  • Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane
  • Primary antibody: Rabbit-anti Human HDAC-1 1:5000
  • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:75000

Detection Level: Low-femtogram

wlo-femto-ultra-web
wlo-femto-ultra-web
  • Two-fold dilution series of HeLa whole cell lysate from 2.5 μg to 0.039 μg of total protein on nitrocellulose membrane
  • Primary antibody:  Rabbit-anti Human HDAC-1 1:10000
  • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:200000

 

Specifications

Application
Western blotting
Brand
Western Lightning ONE
Detection Modality
Chemiluminescence
Sample Type
Chemiluminescent Substrate
Shipping Conditions
Shipped Ambient
Unit Size
25 mL

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