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Multiplex Surefire Ultra p-EGFR (Tyr1068) (Eu) + Total EGFR (Tb) Kit, 10,000 Assay Points

The Alpha SureFire® Ultra™ Multiplex p-EGFR (Tyr1068) + Total EGFR assay kit is used to measure both phosphorylated and total levels of EGFR in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure EGFR (Tyr1068) activation by either recombinant or endogenous receptors, and can be applied to primary cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: MPSU-PTEGFR-K500
Unit Size: 500 Assay Points
Part Number: MPSU-PTEGFR-K10K
Unit Size: 10,000 Assay Points
Part Number: MPSU-PTEGFR-K50K
Unit Size: 50,000 Assay Points
Part Number: MPSU-PTEGFR-K-HV
Unit Size: 100 Assay Points
  • The Alpha SureFire Ultra Multiplex p-EGFR + Total EGFR assay kit is used to measure both phosphorylated and total levels of endogenous EGFR in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated EGFR analysis, and the 545 nm (Tb) signal corresponds to the total EGFR analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

    • No-wash steps, no separation steps
    • Multiplexing assay
    • Sensitive detection
    • No interference from exogenous antibodies
    • Results in less than 3 hours

    In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.

  • Application
    Kinase
    Multiplexing
    Protein Analysis & Detection
    Assay Points
    10000
    Assay Target Class
    Phospho-protein
    Protein
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra Multiplex
    Detection Method
    Alpha
    Shipping Conditions
    Shipped in Blue Ice
    Unit Size
    10,000 Assay Points

Resources

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Brochure
Alpha SureFire Ultra no-wash immunoassay catalog

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Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...

Flyer
Measure more analytes in each well.

Now you can quantify multiple analytes in a single well for faster and more accurate results, even with low sample volumes