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Multiplex Surefire Ultra p-EGFR (Tyr1068) (Eu) + Total EGFR (Tb) Kit, 10,000 Assay Points

The Alpha SureFire® Ultra™ Multiplex p-EGFR (Tyr1068) + Total EGFR assay kit is used to measure both phosphorylated and total levels of EGFR in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure EGFR (Tyr1068) activation by either recombinant or endogenous receptors, and can be applied to primary cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: MPSU-PTEGFR-K500
Unit Size:
Part Number: MPSU-PTEGFR-K10K
Unit Size:
Part Number: MPSU-PTEGFR-K50K
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Part Number: MPSU-PTEGFR-K-HV
Unit Size:
  • The Alpha SureFire Ultra Multiplex p-EGFR + Total EGFR assay kit is used to measure both phosphorylated and total levels of endogenous EGFR in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated EGFR analysis, and the 545 nm (Tb) signal corresponds to the total EGFR analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

    • No-wash steps, no separation steps
    • Multiplexing assay
    • Sensitive detection
    • No interference from exogenous antibodies
    • Results in less than 3 hours

    In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.

  • Application
    Kinase
    Multiplexing
    Protein Analysis & Detection
    Assay Points
    10000
    Assay Target Class
    Phospho-protein
    Protein
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra Multiplex
    Detection Method
    Alpha

Resources

1-3 of 3 Resources
Guide
AlphaPlex quick start guide
alphaplex, guide, overview, assays, technology
Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra
surefire, neutralizing antibody, neutralizing antibodies, biotherapeutics, biotx
Flyer
Measure more analytes in each well.
alphaplex, multiplexing, flyer, acceptor beads, donor beads, beads