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Multiplex Surefire Ultra Phospho-AKT1/2/3 (Ser473 & AKT1 Total) Detection Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra Multiplex phospho-Akt1/2/3(Ser473) + Total Akt1 assay kit is used to measure both the phosphorylation of Akt1/2/3 and total levels of endogenous Akt1 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Akt1/2/3 phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: MPSU-PTAKT-M500
Unit Size: 500 assay points
Part Number: MPSU-PTAKT-M10K
Unit Size: 10,000 Assay Points
Part Number: MPSU-PTAKT-M50K
Unit Size: 50,000 assay points
Part Number: MPSU-PTAKT-M-HV
Unit Size: 100 assay points
  • The Alpha SureFire Ultra Multiplex p-Akt 1/2/3 (Ser473) (Eu) + Total Akt1 (Tb) assay kit is used to measure both phosphorylated and total levels of endogenous Akt in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated Akt1/2/3 analysis, and the 545 nm (Tb) signal corresponds to the total Akt1 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

    • No-wash steps, no separation steps
    • Multiplexing assay
    • Sensitive detection
    • No interference from exogenous antibodies
    • Results in less than 3 hours

    In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.

  • Application
    Protein Analysis & Detection
    Assay Points
    Assay Target Class
    Assay Technology
    Alpha SureFire Ultra Multiplex
    Automation Compatible
    AlphaLISA SureFire Ultra Multiplex
    Detection Method
    Therapeutic Area
    Central Nervous System
    Unit Size
    10,000 Assay Points