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Alpha SureFire Ultra Lysis Buffer B (5X) - 100 mL

Additional Buffer B for select AlphaLISA® SureFire® Ultra assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: ALSU-LBB-10ML
Unit Size: 10 mL
Part Number: ALSU-LBB-100ML
Unit Size: 100 mL
  • Lysis buffer B is already provided in the corresponding AlphaLISA SureFire Ultra kits, used in the detection of phosphoproteins in cell lysates. It is being offered as a standalone item, in case of need of replacement or for additional volume. It is intended to be used solely in conjunction with AlphaLISA SureFire Ultra assays.

    Alpha SureFire Ultra Lysis Buffer B contains generic phosphatase inhibitors but no protease inhibitors. While we usually do not find protease inhibitors necessary, adding such protease inhibitors at standard concentrations will not interfere with the assay. The Alpha SureFire Ultra Lysis Buffer B formulation has been optimized to lyse the cells enough for making accessible the targets to be detected, while not releasing genomic DNA, in order to avoid a too viscous lysate solution that could potentially interfere with the pipetting process. The Alpha SureFire Ultra Lysis Buffer B has been developed to be a generally good buffer for all cell types and targets. Other lysis buffers may be tested, if necessary, but assay performance cannot be guaranteed under such conditions.

  • Application
    Protein Detection
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Unit Size
    100 mL

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