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PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol

PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Imaged on Opera Phenix Plus HCS system.
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Cells were then treated with cytochalasin D and imaged on Opera Phenix Plus HCS system. View All
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Imaged on Opera Phenix Plus HCS system.
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Cells were then treated with cytochalasin D and imaged on Opera Phenix Plus HCS system.
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Imaged on Opera Phenix Plus HCS system.
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Cells were then treated with cytochalasin D and imaged on Opera Phenix Plus HCS system.
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Imaged on Opera Phenix Plus HCS system.
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Cells were then treated with cytochalasin D and imaged on Opera Phenix Plus HCS system.
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Imaged on Opera Phenix Plus HCS system.
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Cells were then treated with cytochalasin D and imaged on Opera Phenix Plus HCS system.

PhenoVue™ Fluor 647 live cell actin stain is a no wash, cell permeable fluorogenic dye which specifically bind to actin filaments and is part of Revvity’s portfolio of cellular imaging reagents. 

PhenoVue Fluor 647 live cell actin stain can be used to visualize actin cytoskeleton in live cells, emiting a far-red fluorescence, and is validated for use in imaging microscopy and high-content screening applications. It exhibits a maximum excitation wavelength at 650 nm and a maximum emission wavelength of 670 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only. Not for use in diagnostic procedures.

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Feature Specification
Color Red
Filter Cy5
Fluorophore PhenoVue™ Fluor 647
Organelle and Cell Compartment Actin
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PhenoVue™ Fluor 647 live cell actin stain is a no wash, cell permeable fluorogenic dye which specifically bind to actin filaments and is part of Revvity’s portfolio of cellular imaging reagents. 

PhenoVue Fluor 647 live cell actin stain can be used to visualize actin cytoskeleton in live cells, emiting a far-red fluorescence, and is validated for use in imaging microscopy and high-content screening applications. It exhibits a maximum excitation wavelength at 650 nm and a maximum emission wavelength of 670 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

For research use only. Not for use in diagnostic procedures.

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
View product information
Product Variants
Quantity: 1 x 5 nmol
Part #:
CP22R1
List Price
USD 420.00
Quantity: 3 x 5 nmol
Part #:
CP22R2
List Price
USD 750.00
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Imaged on Opera Phenix Plus HCS system.
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Cells were then treated with cytochalasin D and imaged on Opera Phenix Plus HCS system.
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Imaged on Opera Phenix Plus HCS system.
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Cells were then treated with cytochalasin D and imaged on Opera Phenix Plus HCS system.
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Imaged on Opera Phenix Plus HCS system.
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Cells were then treated with cytochalasin D and imaged on Opera Phenix Plus HCS system.
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Imaged on Opera Phenix Plus HCS system.
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Cells were then treated with cytochalasin D and imaged on Opera Phenix Plus HCS system.
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Imaged on Opera Phenix Plus HCS system.
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Cells were then treated with cytochalasin D and imaged on Opera Phenix Plus HCS system.
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Imaged on Opera Phenix Plus HCS system.
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Cells were then treated with cytochalasin D and imaged on Opera Phenix Plus HCS system.

Product information

Overview

PhenoVue™ Fluor 647 live cell actin stain is a no wash, cell permeable fluorogenic dye which specifically bind to actin filaments (F-actin) in live cells. Sensitive, rapid and photostable, PhenoVue Fluor 647 live cell actin stain exhibits far-red emission and can be multiplexed with blue, green and orange colors such as PhenoVue nuclear, lysosomal, mitochondrial or tubulin stains. 

Like other actin stains derived from jasplakinolide, cytotoxicity can be observed with long exposure time (>24h) which can be significantly limited at concentrations comprised between 30 and 300nM while maintaining high brightness and image quality.

Depending on the cellular model, intracellular retention of PhenoVue Fluor 647 live cell actin stain can be further improved in the presence of efflux pump inhibitor such as PhenoVue Probenecid, Ready to Use Solution.

Features:

  • Numbers of Vials Per Unit : 1
  • Quantity or Volume Per Vial: 5 nmoles
  • Form: Liquid (DMSO)
  • Storage: -16 °C
  • Recommended Working Concentration: 100 nM
  • Maximum Excitation Wavelength: 650 nm
  • Maximum Emission Wavelength: 670 nm
  • Common Filter Set: Cy5
  • Live Cell Staining: Yes
  • Fixed Cell Staining: Yes
  • Equivalent Number of Microplates:
    • 1 to 5 x 96-well microplates
    • 1 to 5 x 384-well microplates
    • 2 to 8 x 1536-well microplates

Specifications

Color
Red
Form
Solution in DMSO
Maximum Emission Wavelength (Emmax)
670 nm
Maximum Excitation Wavelength (Exmax)
650 nm
Application
High Content Imaging
Microscopy
Brand
PhenoVue™
Detection Modality
Fluorescence
Filter
Cy5
Fluorophore
PhenoVue™ Fluor 647
Organelle and Cell Compartment
Actin
Quantity
1 x 5 nmol
Sample Type
Live and fixed samples
Shipping Conditions
Shipped in Dry Ice
Storage Conditions
-16 °C or below, protected from light
Type
Individual Reagent

Image gallery

PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Imaged on Opera Phenix Plus HCS system.
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Cells were then treated with cytochalasin D and imaged on Opera Phenix Plus HCS system.
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Imaged on Opera Phenix Plus HCS system.
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Cells were then treated with cytochalasin D and imaged on Opera Phenix Plus HCS system.
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Imaged on Opera Phenix Plus HCS system.
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Cells were then treated with cytochalasin D and imaged on Opera Phenix Plus HCS system.
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Imaged on Opera Phenix Plus HCS system.
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Cells were then treated with cytochalasin D and imaged on Opera Phenix Plus HCS system.
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Imaged on Opera Phenix Plus HCS system.
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Cells were then treated with cytochalasin D and imaged on Opera Phenix Plus HCS system.
PhenoVue Fluor 647 Live Cell Actin Stain - 1x5nmol
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Imaged on Opera Phenix Plus HCS system.
U2OS cells stained with PhenoVue Hoechst 33342 (blue) + PhenoVue Fluor 647 live cell actin stain (red) + PhenoVue Fluor 555 live cell tubulin stain (orange). Cells were then treated with cytochalasin D and imaged on Opera Phenix Plus HCS system.

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Resources

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PhenoVue Cellular Imaging Reagents Brochure
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PhenoVue Fluor Live Cell Actin Stain

This is a product information sheet for PhenoVue Fluor live cell actin stain. View validation data, product information, protocols...

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