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PhenoVue Fluor 488 - Concanavalin A
PhenoVue Fluor 488 - Concanavalin A
PhenoVue Fluor 488 - Concanavalin A is a fluorescent lectin which displays high affinity for glycoproteins and glycolipids present at the cellular membranes. It can be used for cellular membrane staining, particularly the endoplasmic reticulum.
PhenoVue Fluor 488 - Concanavalin A exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.
Part of Revvity's portfolio of cellular imaging reagents, PhenoVue Fluor 488 - Concanavalin A has a maximum excitation wavelength of 495 nm and a maximum emission wavelength of 520 nm.
View our extensive validation data in the Product Information Sheet within the Resources tab below.
For research use only. Not for use in diagnostic procedures.
| Feature | Specification |
|---|---|
| Color | Green |
| Filter | FITC |
| Fluorophore | PhenoVue™ Fluor 488 |
| Organelle and Cell Compartment |
Endoplasmic Reticulum Plasma membranes |
PhenoVue Fluor 488 - Concanavalin A is a fluorescent lectin which displays high affinity for glycoproteins and glycolipids present at the cellular membranes. It can be used for cellular membrane staining, particularly the endoplasmic reticulum.
PhenoVue Fluor 488 - Concanavalin A exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.
Part of Revvity's portfolio of cellular imaging reagents, PhenoVue Fluor 488 - Concanavalin A has a maximum excitation wavelength of 495 nm and a maximum emission wavelength of 520 nm.
View our extensive validation data in the Product Information Sheet within the Resources tab below.
For research use only. Not for use in diagnostic procedures.
PhenoVue Fluor 488 - Concanavalin A
PhenoVue Fluor 488 - Concanavalin A
Product information
Overview
Concanavalin A is a plant homotetrameric lectin known to activate the immune system or induce apoptosis and autophagy. Concanavalin A displays high affinity for α-mannopyranosyl and α-glucopyranosyl residues of glycoproteins and glycolipids present at the cellular membranes.
Fluorescent Concanavalin A derivatives are commonly used for staining the cellular membranes of mammalian cells, particularly the endoplasmic reticulum.
PhenoVue Fluor 488 - Concanavalin A can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
Additional product information
Features
| Numbers of vials per unit | 5 |
|---|---|
| Quantity or volume per vial | 1mg (29.2nmoles) |
| Form | Lyophilized |
| Storage | 2-8°C |
| Recommended working concentration | 1mg (9.62nmoles) |
| Maximum excitation wavelength | 495 nm |
| Maximum emission wavelength | 520 nm |
| Common filter set | FITC |
| Live cell staining | Yes |
| Fixed cell staining | Yes. See Product Information Sheet for more information |
| Equivalent number of microplates | 1-5 x 96-well microplates 1-5 x 384-well microplates 3-8 x 1536-well microplates |
Specifications
| Color |
Green
|
|---|---|
| Form |
Lyophilized
|
| Maximum Emission Wavelength (Emmax) |
520 nm
|
| Maximum Excitation Wavelength (Exmax) |
495 nm
|
| Application |
High Content Imaging
Microscopy
|
|---|---|
| Brand |
PhenoVue™
|
| Detection Modality |
Fluorescence
|
| Filter |
FITC
|
| Fluorophore |
PhenoVue™ Fluor 488
|
| Organelle and Cell Compartment |
Endoplasmic Reticulum
Plasma membranes
|
| Quantity |
5 x 1 mg
|
| Sample Type |
Live and fixed samples
|
| Shipping Conditions |
Shipped Ambient
|
| Storage Conditions |
2-8 °C, protected from light
|
| Type |
Individual Reagent
|
Spectra Viewer
Resources
Are you looking for resources, click on the resource type to explore further.
This flyer describes Revvity's PhenoVue cellular imaging reagents.
Fluorescent Concanavalin A conjugates represent a method of choice for labelling the cellular membranes of mammalian cells...
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