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Positive control lysate for AlphaLISA® SureFire® Ultra™ Multiplex Phospho-Rb (Thr821/826)/Total Rb Detection Kit.
The positive control lysate is prepared from Jurkat cells cultured in T175 flasks to approximately 1X106 cells/mL in 10% FBS containing medium, then treated with 1.25 mM Pervanadate for 30 minutes and lysed at 3.2 x 106 cells/mL with 1X SureFire Ultra Lysis buffer.
AlphaLISA SureFire Ultra Multiplex