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Multiplex Surefire Ultra Human Phospho-H2A.X (Ser139)/Total H2A.X Detection Kit, 100 Assay Points

The AlphaLISA® SureFire® Ultra Multiplex Human Phospho-H2A.X (Ser139)/Total H2A.X assay kit is used to measure both the phosphorylation of H2A.X and total levels of endogenous H2A.X in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure H2A.X phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: MPSU-PTH2X-K500
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Part Number: MPSU-PTH2X-K10K
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Part Number: MPSU-PTH2X-K50K
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Part Number: MPSU-PTH2X-K-HV
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  • H2A histone family member X (H2AX) is a type of histone protein encoded by the H2AFX gene. H2AX phosphorylation on serine 139 may occur when double-stranded DNA breaks, either due to ionizing radiation or to endogenous physiological processes. This modification, called gamma-H2AX (phospho-Ser139), triggers cell cycle arrest and DNA damage response repair. Analyzing H2AX allows for a better understanding of genome stability, cell cycle, DNA repair, and more broadly, H2AX is a biomarker for cancer and for aging process investigations.

    The AlphaLISA SureFire Ultra Multiplex Human Phospho-H2A.X (Ser139)/Total H2A.X Detection Kit is used to measure phosphorylated H2A.X (Ser139) and total levels of H2A.X in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated H2A.X, and the 545 nm (Tb) signal corresponds to the total H2A.X analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

    • No-wash steps, no separation steps
    • Multiplexing assay
    • Sensitive detection
    • No interference from exogenous antibodies
    • Results in less than 3 hours

    In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2 agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.

  • Application
    Protein Detection
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra Multiplex
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Therapeutic Area
    Oncology

Resources

1-3 of 3 Resources
Guide
AlphaPlex quick start guide
alphaplex, guide, overview, assays, technology
Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra
surefire, neutralizing antibody, neutralizing antibodies, biotherapeutics, biotx
Flyer
Measure more analytes in each well.
alphaplex, multiplexing, flyer, acceptor beads, donor beads, beads