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Multiplex Surefire Ultra Human Phospho-CDK1 (Tyr15)/Total CDK1 Detection Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra Human Phospho-CDK1 (Tyr15)/Total CDK1 assay kit is used to measure both the phosphorylation of CDK1 (Tyr15) and total levels of endogenous CDK1 in cellular lysates.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: MPSU-PTCDK1-M500
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Part Number: MPSU-PTCDK1-M10K
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Part Number: MPSU-PTCDK1-M50K
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Part Number: MPSU-PTCDK1-M-HV
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  • Cyclin-dependent kinase 1 (CDK1), encoded by the CDC2 gene, is a serine/threonine kinase ubiquitously expressed in eukaryotes. CDK1 is a catalytic subunit of a protein kinase called the M-phase promoting factor that induces entry into mitosis. Dysregulated activation of CDK1 leads to uncontrolled cell division and is a hallmark of cancer. Inhibition of CDK1 is an active research area, especially with new approaches involving PROTACs.

    The AlphaLISA SureFire Ultra Multiplex Human Phospho-CDK1 (Tyr15)/Total CDK1 Detection Kit is used to measure phosphorylated CDK1 (Tyr15) and total levels of CDK1 in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated CDK1, and the 545 nm (Tb) signal corresponds to the total CDK1 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

    • No-wash steps, no separation steps
    • Multiplexing assay
    • Sensitive detection
    • No interference from exogenous antibodies
    • Results in less than 3 hours

    In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.

  • Application
    Protein Detection
    Assay Points
    50000
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra Multiplex
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Therapeutic Area
    Oncology

Resources

1-3 of 3 Resources
Guide
AlphaPlex quick start guide
alphaplex, guide, overview, assays, technology
Application Note Icon Image
Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra
surefire, neutralizing antibody, neutralizing antibodies, biotherapeutics, biotx
Flyer
Measure more analytes in each well.
alphaplex, multiplexing, flyer, acceptor beads, donor beads, beads