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Multiplex Surefire Ultra Phospho-AKT1/2/3 (Thr450 & Total) Detection Kit, 100 Assay Points

The AlphaLISA® SureFire® Ultra Human and Mouse phospho-AKT 1/2/3 (Thr450)/Total AKT1 assay kit is used to measure both the phosphorylation of AKT 1/2/3 and total levels of endogenous AKT 1 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure AKT 1/2/3 phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: MPSU-PTAKT-N500
Unit Size: 500 Assay Points
Part Number: MPSU-PTAKT-N10K
Unit Size: 10,000 Assay Points
Part Number: MPSU-PTAKT-N50K
Unit Size: 50,000 Assay Points
Part Number: MPSU-PTAKT-N-HV
Unit Size: 100 Assay Points
  • Protein kinase B (PKB) also known as AKT1 AKT2 and AKT3 encode the RAC alpha, beta, and gamma serine/threonine protein kinases respectively. AKT1 is involved in cellular survival pathways, by inhibiting apoptotic process. It is also able to induce protein synthesis pathways and is therefore a key signaling protein in the cellular pathways that lead to skeletal muscle hypertrophy and general tissue growth. Akt2 is an important signaling molecule in the insulin signaling pathway. AKT3 appears to be predominantly expressed in the brain.

    The AlphaLISA SureFire Ultra Multiplex Human and Mouse Phospho-AKT 1/2/3 (Thr450)/Total AKT1 Detection Kit is used to measure phosphorylated AKT 1/2/3 and total levels of AKT1 in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated AKT 1/2/3, and the 545 nm (Tb) signal corresponds to the total AKT1 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

    • No-wash steps, no separation steps
    • Multiplexing assay
    • Sensitive detection
    • No interference from exogenous antibodies
    • Results in less than 3 hours

    In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.

  • Application
    Protein Detection
    Assay Points
    100
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA SureFire Ultra Multiplex
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Shipping Conditions
    Shipped in Blue Ice
    Target Species
    Human
    Mouse
    Therapeutic Area
    Cardiovascular
    Oncology
    Unit Size
    100 Assay Points

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