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LANCE Ultra Phospho-BTK (Tyr223) Detection Kit, 500 Assay Points

LANCE Ultra phospho-BTK (Tyr223) kits are designed for the detection of phosphorylated BTK in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: TRF4021C
Unit Size: 500 Assay Points
List price: USD 2,043.00
Your price:
USD 2,043.00
USD 2,043.00 /each
Part number: TRF4021M
Unit Size: 10,000 Assay Points
List price: USD 9,232.00
Your price:
USD 0.00
USD 9,232.00 /each

Overview

Please note control lysates are sold separately, catalog number TRF4021S.

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

BTK is a cytoplasmic tyrosine kinase present in B-cells. It is associated with the B-cell receptor and receptors of the toll family. Upon activation, BTK will activate the Nf-kB pathway. This will induce maturation of B-cells into antibody producing cells and also increase the secretion of activating cytokines (such as IL-2). The protein is absent or non-functional in diseases such as aglobularia, but is also involved by hyperactivation in inflammation and auto-immune diseases. Also, several leukemia-like cancers have dysregulation of BTK.

Specifications

Application
Kinase
Protein Analysis & Detection
Assay Points
500
Assay Target Class
Phospho-protein
Automation Compatible
Yes
Brand
LANCE Ultra
Detection Method
Time-Resolved Fluorescence (TRF)
TR-FRET
Shipping Conditions
Shipped in Blue Ice
Therapeutic Area
Autoimmunity
Immuno-oncology
Neuroscience
Oncology
Unit Size
500 Assay Points

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SDS, COAs, Manuals and more

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