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LANCE Ultra CCL3 Detection Kit, 500 Assay Points

The LANCE® Ultra Human CCL3/MIP1α Detection Kit is designed for detection and quantitation of human CCL3 (MIP1α) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: TRF1257C
Unit Size: 500 Assay Points
Part Number: TRF1257M
Unit Size: 10,000 Assay Points
  • The LANCE®Ultra Human CCL3/MIP1α Detection Kit is designed for detection and quantitation of human CCL3 (MIP1α) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

    • No-wash steps, no separation steps
    • TR-FRET technology
    • Sensitive detection
    • High reproducibility
    • Faster time-to-results
    • Easy automation
    • 96-well, 384-well, and 1536-well formats

    LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

    C-C Motif Chemokine 3 (CCL3), also known as Macrophage Inflammatory Protein-1 alpha (MIP-1α), contains 70 amino acids in its mature form. It is a factor produced by macrophages that causes local inflammatory responses in vivo, and induces superoxide production by neutrophils in vitro. CCL3 is involved in the acute inflammatory state and in the recruitment and activation of polymorphonuclear leukocytes. It binds to CCR1, CCR4, and CCR5. CCL3 is known mainly for its activity as a chemotactic cytokine, but may also have a potential role in host defense as a direct antiviral agent. CCL3 has been reported to show a significant direct antiviral activity against HSV-1. It is one of the major HIVsuppressive factors produced by CD8+ T-cells. CCL3 is also an inhibitor of haematopoietic stem cell proliferation.

  • Assay Points
    500
    Assay Target Class
    Cytokine
    Automation Compatible
    Yes
    Brand
    LANCE Ultra
    Detection Method
    Time-Resolved Fluorescence (TRF)
    TR-FRET
    Experimental Type
    In vitro
    Format
    Microplates
    Quantity in a Package Amount
    1.0 Units
    Species
    Human
    Shipping Conditions
    Shipped in Blue Ice
    Therapeutic Area
    Inflammation
    Unit Size
    500 Assay Points