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LabChip High pI Charge Variant Analysis Kit

The LabChip® Charge Variant Analysis Assays allow you to efficiently analyze the charge variant profiles of basic and acidic protein samples.

For research use only. Not for use in diagnostic procedures.

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YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Feature Specification
Instrument Compatibility LabChip GXII Touch

The LabChip® Charge Variant Analysis Assays allow you to efficiently analyze the charge variant profiles of basic and acidic protein samples.

For research use only. Not for use in diagnostic procedures.

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Sample Type: Protein
Type
High pI (7.0 - 9.0)
Part #:
CLS760670
List Price
USD 558.07
Sample Type: Protein
Type
Low pI (3.0 - 7.0)
Part #:
CLS154134
List Price
USD 603.17

Overview

Increase the speed, efficiency, and reliability of charge variant analysis.

All biotherapeutic protein molecules come with charge heterogeneity sites, known as charge variants. Charge variants affect biological functions, such as binding and stability. Charge variant profiles also change dynamically throughout processes, such as sequence expression, chemical degradation, and post-translational modification. It is important to monitor and screen charge variant profiles of biotherapeutic proteins throughout the development and manufacturing processes.

Revvity’s LabChip® charge variant analysis assays are comparable to traditional CZE assays (Han, 2011) and can resolve and screen the charge variant profile of samples quickly, reliably, and automatically. With the throughput capability on the LabChip® GXII Touch™ protein characterization system, users can optimize their valuable research time to accelerate discovery and development of new biotherapeutic solutions.

Additional product information

Obtain speed and efficiency of charge variant analysis throughout biotherapeutic development and manufacturing processes.

  • Simple and automated operational workflow
  • Fast analysis speed: ~60 seconds per sample
  • High throughput: 96 samples per experiment
  • Minimal sample consumption: 25 μL
  • Full pI range coverage: pI 3 – 9, both acidic and basic proteins
  • Protein migration time can be adjusted based on running buffer pH
  • Good linearity: peak intensity proportional to variant concentration
  • Reliable repeatability with C.V. < 2.5 %
labchip charge variant analysis assays fig1

Figure 1. Charge variant profile of a positive charged mAb on the LabChip high pI charge variant assay. Due to a stronger electrophoretic force, basic variants move toward the cathode faster and arrive earlier at the detection window than main variants and acidic variants. Basic, main and acidic variants are separated, resolved and labeled in the LabChip charge variant assay. The relative concentration of each variant was also quantified.

labchip charge variant analysis assays fig2

Figure 2. LabChip Charge Variant Assays measure charge variant profile of protein reliably. Under pH =7.2 running buffer, charge variant profiles of bovine serum albumin exhibit same migration pattern. Migration time of main variant C.V. = 1.29% for two repeatable runs.

Ordering Information
  • Assay: Labchip High pl Charge Variant Analysis
  • Reagent Kit: CLS760670
  • LabChip HT: CLS153419

Specifications

Application
Protein Analysis
Automation Compatible
Yes
Instrument Compatibility
LabChip GXII Touch
Sample Type
Protein
Shipping Conditions
Dual Temperature
Technology
Microfluidic Electrophoresis
Type
High pI (7.0 - 9.0)
Unit Size
1 kit

References

  • Han, H., Livingston, E., and Chen, X. (2011) High Throughput Profiling of Charge Heterogeneity in Antibodies by Microchip Electrophoresis Anal. Chem. 83, 21, 8184–8191. doi.org/10.1021/ac201741w.

Resources

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Product Note
Automated characterization of proteins and nucleic acids

Read the LabChip® GXII Touch protein characterization system product note to learn about an automated alternative to traditional...

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