Obtain speed and efficiency of charge variant analysis throughout biotherapeutic development and manufacturing processes.

• Simple and automated operational workflow
• Fast analysis speed: ~60 seconds per sample
• High throughput: 96 samples per experiment
• Minimal sample consumption: 25 μL
• Full pI range coverage: pI 3 – 9, both acidic and basic proteins
• Protein migration time can be adjusted based on running buffer pH
• Good linearity: peak intensity proportional to variant concentration
• Reliable repeatability with C.V. < 2.5 %

Figure 1. Charge variant profile of a positive charged mAb on the LabChip high pI charge variant assay. Due to a stronger electrophoretic force, basic variants move toward the cathode faster and arrive earlier at the detection window than main variants and acidic variants. Basic, main and acidic variants are separated, resolved and labeled in the LabChip charge variant assay. The relative concentration of each variant was also quantified.

Figure 2. LabChip Charge Variant Assays measure charge variant profile of protein reliably. Under pH =7.2 running buffer, charge variant profiles of bovine serum albumin exhibit same migration pattern. Migration time of main variant C.V. = 1.29% for two repeatable runs.

Ordering Information

• Assay: Labchip High pl Charge Variant Analysis
• Reagent Kit: CLS760670
• LabChip HT: CLS153419

• Han, H., Livingston, E., and Chen, X. (2011) High Throughput Profiling of Charge Heterogeneity in Antibodies by Microchip Electrophoresis Anal. Chem. 83, 21, 8184–8191. doi.org/10.1021/ac201741w.