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LabChip High pI Charge Variant Analysis

The LabChip® Charge Variant Analysis Assays allow you to efficiently analyze the charge variant profiles of basic and acidic protein samples.

For research use only. Not for use in diagnostic procedures.
Part Number: CLS760670
Type: High pI (7.0 - 9.0)
Part Number: CLS154134
Type: Low pI (3.0 - 7.0)
  • Increase the speed, efficiency, and reliability of charge variant analysis.

    All biotherapeutic protein molecules come with charge heterogeneity sites, known as charge variants. Charge variants affect biological functions, such as binding and stability. Charge variant profiles also change dynamically throughout processes, such as sequence expression, chemical degradation, and post-translational modification. It is important to monitor and screen charge variant profiles of biotherapeutic proteins throughout the development and manufacturing processes.

    Revvity’s LabChip® charge variant analysis assays are comparable to traditional CZE assays (Han, 2011) and can resolve and screen the charge variant profile of samples quickly, reliably, and automatically. With the throughput capability on the LabChip® GXII Touch protein characterization system, users can optimize their valuable research time to accelerate discovery and development of new biotherapeutic solutions.

  • Obtain speed and efficiency of charge variant analysis throughout biotherapeutic development and manufacturing processes.

    • Simple and automated operational workflow
    • Fast analysis speed: ~60 seconds per sample
    • High throughput: 96 samples per experiment
    • Minimal sample consumption: 25 μL
    • Full pI range coverage: pI 3 – 9, both acidic and basic proteins
    • Protein migration time can be adjusted based on running buffer pH
    • Good linearity: peak intensity proportional to variant concentration
    • Reliable repeatability with C.V. < 2.5 %
    labchip charge variant analysis assays fig1

    Figure 1. Charge variant profile of a positive charged mAb on the LabChip high pI charge variant assay. Due to a stronger electrophoretic force, basic variants move toward the cathode faster and arrive earlier at the detection window than main variants and acidic variants. Basic, main and acidic variants are separated, resolved and labeled in the LabChip charge variant assay. The relative concentration of each variant was also quantified.

    labchip charge variant analysis assays fig2

    Figure 2. LabChip Charge Variant Assays measure charge variant profile of protein reliably. Under pH =7.2 running buffer, charge variant profiles of bovine serum albumin exhibit same migration pattern. Migration time of main variant C.V. = 1.29% for two repeatable runs.

    Ordering Information
    • Assay: Labchip High pl Charge Variant Analysis
    • Reagent Kit: CLS760670
    • LabChip HT: CLS153419
  • Analytical Technique
    Protein Analysis & Detection
    Application
    Protein Analysis
    Brand
    LabChip GXII
    Quantity in a Package Amount
    1.0 Units
    Research Areas
    Drug Development & Manufacturing
    Drug Discovery & Manufacturing
    Food & Agriculture
    Sample Type
    Protein
    Shipping Conditions
    Shipped in Dry Ice
    Type
    High pI (7.0 - 9.0)
    Unit Size
    1 kit
    • Han, H., Livingston, E., and Chen, X. (2011) High Throughput Profiling of Charge Heterogeneity in Antibodies by Microchip Electrophoresis Anal. Chem. 83, 21, 8184–8191. doi.org/10.1021/ac201741w.