The HT DNA 5K/HT RNA/ Charge Variant Assay LabChip is designed for use with the LabChip HT DNA 5K, HT RNA, charge variant assay, and the RNA Pico assays on the LabChip GX and GXII systems.
For research use only. Not for use in diagnostic procedures.
Feature | Specification |
---|---|
Analytical Sensitivity | DNA 0.25 ng/µL, Pico RNA 250 pg/µL Total RNA, Small RNA 50 pg/μL, 500 pg/µL mRNA |
Application |
DNA Analysis Protein Analysis |
Linear Concentration Range | DNA 0.25 - 50 ng/µL 0.2 per fragment, RNA 25 - 250 ng/ μL (Std Sens) and 5 - 50 ng/μL (High Sens), Pico RNA 500 - 5,000 pg/µL Total RNA 625 - 5,000 pg/µL mRNA, small RNA 100 - 10,000 pg/µL, Protein 25 µL with conc. between 0.5 - 10 mg/mL |
Size Range | DNA 100 - 5,000 bp, RNA 100 - 6,000 nt, Small RNA 20 - 150 nt |
Sizing Precision RSD (CV) | DNA ± 10%, small RNA CV < 5.0% or 4 nt, Protein CV < 5% for varying concentration from 1 - 3 mg/mL CV < 3% at constant concentration |
Sizing Resolution | DNA ± 10% from 150 - 500 bp ± 15% from 100 - 150 bp, 500 - 1500 bp ± 20% from 1500 - 5000 bp, Protein Comparable to IEX and conventional CZE |
Feature | Specification |
---|---|
Analytical Sensitivity | DNA 0.25 ng/µL, Pico RNA 250 pg/µL Total RNA, Small RNA 50 pg/μL, 500 pg/µL mRNA |
Application |
DNA Analysis Protein Analysis |
Linear Concentration Range | DNA 0.25 - 50 ng/µL 0.2 per fragment, RNA 25 - 250 ng/ μL (Std Sens) and 5 - 50 ng/μL (High Sens), Pico RNA 500 - 5,000 pg/µL Total RNA 625 - 5,000 pg/µL mRNA, small RNA 100 - 10,000 pg/µL, Protein 25 µL with conc. between 0.5 - 10 mg/mL |
Size Range | DNA 100 - 5,000 bp, RNA 100 - 6,000 nt, Small RNA 20 - 150 nt |
Sizing Precision RSD (CV) | DNA ± 10%, small RNA CV < 5.0% or 4 nt, Protein CV < 5% for varying concentration from 1 - 3 mg/mL CV < 3% at constant concentration |
Sizing Resolution | DNA ± 10% from 150 - 500 bp ± 15% from 100 - 150 bp, 500 - 1500 bp ± 20% from 1500 - 5000 bp, Protein Comparable to IEX and conventional CZE |
The HT DNA 5K/HT RNA/ Charge Variant Assay LabChip provides a fast and easy way to analyze DNA, RNA, and to preform charge variant analysis of monoclonal antibody (mAb) samples (if using V1.9 software or older). If using newer software, use the High pI Charge Variant LabChip (P/N CLS153419) for charge variant analysis instead.Performing DNA, RNA sample analysis with the LabChip electrophoresis system saves time and money by automating the steps for analyzing size and purity, replacing agarose slab gels and imaging. Chips are prepped once per day, supporting a variety of samples workflows, have a long sample life, and they can be reused multiple days to lower your per sample costs.
Analytical Sensitivity |
DNA 0.25 ng/µL, Pico RNA 250 pg/µL Total RNA, Small RNA 50 pg/μL, 500 pg/µL mRNA
|
---|---|
Application |
DNA Analysis
Protein Analysis
|
Automation Compatible |
Yes
|
Instrument Compatibility |
LabChip GXII Touch
LabChip GX Touch
|
Linear Concentration Range |
DNA 0.25 - 50 ng/µL 0.2 per fragment, RNA 25 - 250 ng/ μL (Std Sens) and 5 - 50 ng/μL (High Sens), Pico RNA 500 - 5,000 pg/µL Total RNA 625 - 5,000 pg/µL mRNA, small RNA 100 - 10,000 pg/µL, Protein 25 µL with conc. between 0.5 - 10 mg/mL
|
Sample Type |
DNA
Protein
RNA
|
Sample Volume |
DNA 2 μL, RNA 2 μL RNA Std Sens or 6 μL RNA High Sens. Pico RNA 2 μL, Small RNA 2 μL, Protein 25 μL
|
Size Range |
DNA 100 - 5,000 bp, RNA 100 - 6,000 nt, Small RNA 20 - 150 nt
|
Sizing Precision RSD (CV) |
DNA ± 10%, small RNA CV < 5.0% or 4 nt, Protein CV < 5% for varying concentration from 1 - 3 mg/mL CV < 3% at constant concentration
|
Shipping Conditions |
Shipped in Blue Ice Soft
|
Sizing Resolution |
DNA ± 10% from 150 - 500 bp ± 15% from 100 - 150 bp, 500 - 1500 bp ± 20% from 1500 - 5000 bp, Protein Comparable to IEX and conventional CZE
|
Storage Conditions |
4°C
|
Technology |
Microfluidic Electrophoresis
|
Throughput |
DNA 28 sec
Protein 90 - 110 sec
RNA 80 sec
Small RNA 80 sec
|
Unit Size |
1 chip
|
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