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IVIS Lumina X5 Imaging System

High-Sensitivity Optical Meets High-Resolution X-ray

The IVIS® Lumina X5 high-throughput 2D optical imaging system combines high-sensitivity bioluminescence and fluorescence with high-resolution x-ray into a compact system that fits on your benchtop. With an expanded 5 mouse field of view for 2D optical imaging plus our unique line of accessories to accelerate setup and labeling, it has never been easier or faster to get robust data- and answers- on anatomical and molecular aspects of disease.

For research use only. Not for use in diagnostic procedures.


The IVIS Lumina X5 has all the capabilities of the IVIS Lumina S5 imaging system with integrated industry leading high-resolution x-ray for greater detail. The IVIS Lumina X5 also includes state of the art spectral unmixing features for sensitive multispectral imaging to monitor multiple biological events in the same animal.

High-throughput Optical and X-ray Imaging – No Compromise

The IVIS Lumina X5 integrates a 1 inch CCD camera into our benchtop Lumina instrument providing a high throughput 20 x 20 cm FOV sufficient for imaging 5 animals at a time with bioluminescence and fluorescence. Moreover, the large, independently deployed scintillator facilitates X-ray acquisitions of 5 mice and larger rodents up to 500-600 grams with seamless, accurate overlay onto the optical image at any field of view.

As with other IVIS Lumina systems, the X5 is equipped with 26 filters tunable to image fluorescent sources that emit from green to near-infrared. Novel illumination technology effectively increases fluorescent transmission through 900 nm. Additionally, the IVIS Lumina X5 incorporates Revvity's patented Compute Pure Spectrum (CPS) algorithm for spectral library generation software tools to ensure accurate autofluorescence removal, unmixing and fluorophore quantitation.

Standard on all IVIS instruments, absolute calibration affords consistent and reproducible results independent of magnification, filter selection from one instrument to any another IVIS instrument within an organization or around the world.

Industry Leading X-ray Resolution

The IVIS Lumina X5 is equipped with a microfocus X-ray source and geometric magnification that when combined achieve industry leading X-ray resolution in a 2D optical/X-ray system. This sets a new standard in multimodal 2D imaging resolution. With optical image overlays at every X-ray resolution, never miss underlying anatomical and structural changes. Get more from your data and explore new applications.

IVIS Lumina X5 – A High Throughput Solution

Not only does the IVIS Lumina X5 offer higher throughput via the 1 inch CCD, but it is also compatible with a set of smart animal handling accessories (purchased separately) designed with throughput and safety in mind.

Smart loading trays will allow users to pose animals on the benchtop before placing the tray into the IVIS. Fiducials built into the tray will allow the software to automatically recognize and draw ROIs providing automated animal identification.

Animal trays are designed with ease of use and user safety in mind. No nose cones are required thus minimizing cleanup. All tray parts are autoclaveable for ease of sterilization and when used with the next generation anesthesia unit (RAS-4), strong vacuum capabilities minimize excess gas from escaping thus preventing exposure of users to anesthetic gas.

Finally, Living Image® software brings IVIS technology to life by facilitating an intuitive workflow for in vivo optical, X-ray image acquisition, analysis and data organization. The software’s design creates an intuitive, seamless workflow for researchers of all skill levels. Living Image will support input of unique animal IDs when using chip technologies and readers from third party vendors thus streamlining labeling, setup and subsequent export of data for analysis.

Key Features:

  • High throughput (5 mouse) optical and X-ray
  • High resolution, low dose X-ray with optical overlay
  • Supports mouse and rat imaging
  • Compute Pure Spectrum (CPS) spectral unmixing
  • Full fluorescence tunability through the NIR spectrum
  • Unique accessories to streamline workflow, data acquisition and analysis


116.84 cm
48.26 cm
Imaging Modality
Optical Imaging

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  • Zhou et al (2021). Dipeptidyl Peptidase-4 modulates Long-chain Acyl-CoA synthetase 4 to Promote Lipid Peroxidation and Regulate Ferroptosis in Pancreatic Cancer. Res Square.
  • Rupp et al (2021). Therapeutic potential of Fingolimod in triple negative breast cancer preclinical models. Translational Oncology. 14(1): 100926.
  • Jiang et al (2020). Combined Treatment With CCR1-Overexpressing Mesenchymal Stem Cells and CCL7 Enhances Engraftment and Promotes the Recovery of Simulated Birth Injury-Induced Stress Urinary Incontinence in Rats. Fontiers in Surgery. 7:40.
  • Trikha et al (2020). Inhibition of Angiotensin Converting Enzyme Impairs Anti-staphylococcal Immune Function in a Preclinical Model of Implant Infection. Front. Immunol.
  • Brandon et al (2020). Identification of ovarian high-grade serous carcinoma cell lines that show estrogen-sensitive growth as xenografts in immunocompromised mice. Sci Reports. 10: 10799.
  • Kelley et al (2020). In vivo Mouse Model of Spinal Implant Infection. Medicine.
  • Lefeuvre et al (2020). Effects of topical corticosteroids and lidocaine on Borrelia burgdorferi sensu lato in mouse skin: potential impact to human clinical trials. Sci Reports. 10: 10552.
  • Liang et al (2019). Idarubicin-loaded methoxy poly(ethylene glycol)-b-poly(l-lactide-co-glycolide) nanoparticles for enhancing cellular uptake and promoting antileukemia activity. Int J Nanomedicine. 14: 543–556.
  • Karches et al (2019). Bispecific Antibodies Enable Synthetic Agonistic Receptor-Transduced T Cells for Tumor Immunotherapy. Translational Cancer Mechanisms and Therapy.


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