US
Search Results
Revvity Sites Globally
Select your location.
*e-commerce not available for this region.
HTRF Testosterone Detection Kit, 500 Assay Points
The Testosterone kit is designed for the fast quantification of the steroid hormone in cell-based and enzymatic formats.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Product information
Overview
Testosterone is a steroid hormone synthesized from 17a-hydroxy progesterone by the enzymes 17,20-lyase and 17ß-hydroxysteroid dehydrogenase in the gonads of both males and females, although small amounts are also secreted by the adrenal glands. It is the principal male sex hormone and an anabolic steroid. It plays an important role in the stimulation and the development of masculine characteristics by its fixation of androgen receptors.
Specifications
| Assay Points |
500
|
|---|---|
| Assay Target Type |
Kit
|
| Assay Technology |
HTRF
|
| Brand |
HTRF
|
| Quantity |
1
|
| Therapeutic Area |
Metabolism/Diabetes
Neuroscience
|
| Unit Size |
500 Assay Points
|
Video gallery
How it works
Assay principle
The testosterone assay is based on the competition principle, where native testosterone produced by cells and XL665-labelled testosterone compete for binding to a monoclonal anti-testosterone antibody labelled with Europium Cryptate.
Assay Protocol
The testosterone assay features a streamlined protocol with only two incubation steps: - Cell stimulation by target compounds - Testosterone detection using HTRF reagents This protocol requires a single one-hour incubation period following cell stimulation.
Assay details
Key features
| Detection limit: | 0.1 nM |
| Dynamic range: | 0.1 to 10 nM |
| EC50 | 0.84 nM |
| S/B | 13 |
| Specificity: | low cross-reactivity with 4-Androstene-3,17-Dione 1.3% |
Resources
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
SDS, COAs, Manuals and more
Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.
How can we help you?
We are here to answer your questions.
