Skip to main content

HTRF Testosterone Detection Kit, 500 Assay Points

The Testosterone kit is designed for the fast quantification of the steroid hormone in cell-based and enzymatic formats.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: 64TESPEG
Unit Size: 500 Assay Points
Part Number: 64TESPEH
Unit Size: 10,000 Assay Points
  • Testosterone is a steroid hormone synthesized from 17a-hydroxy progesterone by the enzymes 17,20-lyase and 17ß-hydroxysteroid dehydrogenase in the gonads of both males and females, although small amounts are also secreted by the adrenal glands. It is the principal male sex hormone and an anabolic steroid. It plays an important role in the stimulation and the development of masculine characteristics by its fixation of androgen receptors.

  • Assay Points
    500
    Assay Target Type
    Kit
    Assay Technology
    HTRF
    Brand
    HTRF
    Quantity
    1
    Therapeutic Area
    Metabolism/Diabetes
    Neuroscience
    Unit Size
    500 Assay Points
  • Assay principle

    The testosterone assay is based on the competition principle, where native testosterone produced by cells and XL665-labelled testosterone compete for binding to a monoclonal anti-testosterone antibody labelled with Europium Cryptate.

    Biomarkers assay principle testosterone
    Assay Protocol

    The testosterone assay features a streamlined protocol with only two incubation steps: - Cell stimulation by target compounds - Testosterone detection using HTRF reagents This protocol requires a single one-hour incubation period following cell stimulation.

    Assay protocol testosterone

     

  • Key features
    Detection limit: 0.1 nM
    Dynamic range: 0.1 to 10 nM
    EC50 0.84 nM
    S/B 13
    Specificity: low cross-reactivity with
    4-Androstene-3,17-Dione 1.3%
    Specifications testosterone

     

Resources

1-1 of 1 Resources
Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.