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HTRF Tau Aggregation Kit, 500 Assay Points

The Tau Aggregation kit is designed for the fast detection of human Tau aggregates in brain tissues and cell cultures.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Protein Quantification
Sample Volume 10 µL

The Tau Aggregation kit is designed for the fast detection of human Tau aggregates in brain tissues and cell cultures.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 500 Assay Points
Part #:
6FTAUPEG
List Price
USD 1,210.00
Unit Size: 10,000 Assay Points
Part #:
6FTAUPEH
List Price
USD 14,380.00

Overview

Tau proteins are required to stabilize microtubules through tubulin association. Deregulation of Tau expression, aggregation, and subsequent phosphorylation have been correlated to the onset of pathologies linked to the nervous system such as Alzheimer's and Parkinson's. The Tau aggregation assay detects human Tau aggregates rapidly in brain tissues and cell cultures. This kit is part of our Tau range, including phosphorylated Tau detection and total Tau measurement (MAP-Tau).

Specifications

Application
Protein Quantification
Brand
HTRF
Detection Modality
HTRF
Product Group
Kit
Sample Volume
10 µL
Shipping Conditions
Shipped in Dry Ice
Target Class
Biomarkers
Technology
TR-FRET
Therapeutic Area
Neuroscience
Unit Size
500 Assay Points

Video gallery

Citations

How it works

Assay principle

Tau aggregates are measured using a sandwich immunoassay involving an anti-Tau monoclonal antibody specific for Tau aggregate labelled with terbium-Cryptate or d2, ensuring assay quality, reproducibility, and signal quality. The specific HTRF signal generated is proportional to the Tau aggregates.

Biomarkers assay principle tau

 

Assay Protocol

The sample or brain extracts are transferred to the assay plate for the detection of Tau aggregates. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.

Assay protocol tau

Assay details

Assay specificity & linearity

A FRET signal is generated in the presence of aggregated tau, leading to a distinctive signal compared to non-aggregated tau. The assay shows a good linear correlation between signal intensity and the purified recombinant tau aggregate concentrations obtained by serial dilutions in assay diluent, with a linear range of 1.9 to 500 ng/mL.

Specifications tau aggregation

Assay validation

Tau aggregation kinetic study

Recombinant full-length human tau protein was chemically aggregated. The reaction was stopped at different times (1, 2, 4, 6 and 24 h). The samples were previously diluted (1/500) to follow the kinetics of aggregation. This assay could also be used to monitor a kinetic of dissociation after a compound addition. The test was performed in 384-well low volume plates, read on a PHERAstar FS laser.

Assay validation tau aggregation

Resources

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Literature - Publication Review
Tau aggregation in Alzheimer's disease pathogenesis addressed with HTRF assays

See how peer researchers challenge tau aggregation with HTRF

This literature review illustrates the possibilities and versatility...

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