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HTRF Tau Aggregation aggregation Detection Kit, 500 Assay Points

The Tau Aggregation kit is designed for the fast detection of human Tau aggregates in brain tissues and cell cultures.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: 6FTAUPEG
Unit Size: 500 Assay Points
Part Number: 6FTAUPEH
Unit Size: 10,000 Assay Points
  • Tau proteins are required to stabilize microtubules through tubulin association. Deregulation of Tau expression, aggregation, and subsequent phosphorylation have been correlated to the onset of pathologies linked to the nervous system such as Alzheimer's and Parkinson's. The Tau aggregation assay detects human Tau aggregates rapidly in brain tissues and cell cultures. This kit is part of our Tau range, including phosphorylated Tau detection and total Tau measurement (MAP-Tau).

  • Assay Points
    500
    Assay Target Type
    Kit
    Assay Technology
    HTRF
    Brand
    HTRF
    Quantity
    1
    Therapeutic Area
    Neuroscience
    Unit Size
    500 Assay Points
  • Assay principle

    Tau aggregates are measured using a sandwich immunoassay involving an anti-Tau monoclonal antibody specific for Tau aggregate labelled with terbium-Cryptate or d2, ensuring assay quality, reproducibility, and signal quality. The specific HTRF signal generated is proportional to the Tau aggregates.

    Biomarkers assay principle tau

     

    Assay Protocol

    The sample or brain extracts are transferred to the assay plate for the detection of Tau aggregates. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.
     

    Assay protocol tau

     

  • Assay specificity & linearity

    A FRET signal is generated in the presence of aggregated tau, leading to a distinctive signal compared to non-aggregated tau. The assay shows a good linear correlation between signal intensity and the purified recombinant tau aggregate concentrations obtained by serial dilutions in assay diluent, with a linear range of 1.9 to 500 ng/mL.

    Specifications tau aggregation

     

  • Tau aggregation kinetic study

    Recombinant full-length human tau protein was chemically aggregated. The reaction was stopped at different times (1, 2, 4, 6 and 24 h). The samples were previously diluted (1/500) to follow the kinetics of aggregation. This assay could also be used to monitor a kinetic of dissociation after a compound addition. The test was performed in 384-well low volume plates, read on a PHERAstar FS laser.

    Assay validation tau aggregation

     

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Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.