Skip to main content

HTRF Tag-Lite CXCR4 (HEK293) Receptor Cell line

This cell line stably expresses the CXCR4 receptor fused to a SNAP-Tag, and can be used in a Tag-lite application.

For research use only. Not for use in diagnostic procedures.
Part Number: C1SU1CXCR4
Part Number: C1SU1CXCR4
Unit Size: 1 Item
  • CXCR4 is a receptor for the C-X-C chemokine CXCL12/SDF-1. Compelling evidences suggests that CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation. The receptor also plays an essential role in vascularization of the gastrointestinal tract. CXCR4 acts as a coreceptor for human immunodeficiency virus-1/HIV-1.

    This HEK293 stable cell line expresses the CXCR4 receptor fused to a SNAP-Tag. Labeled with Terbium, the cells can be used in a Tag-lite receptor binding protocol.

  • Assay Points
    1
    Assay Technology
    HTRF
    Brand
    Tag-Lite
    Quantity
    1
    Shelf Life
    3650.0 Day(s)
    Shipping Conditions
    Shipped in Dry Ice
    Therapeutic Area
    Cardiovascular
    Infectious Diseases
    Metabolism/Diabetes
    NASH/Fibrosis
    Neuroscience
    Oncology & Inflammation
    Rare Diseases
    Unit Size
    1 Item
  • Step 1 - Receptor labeling

    SNAP-tag® is a small fusion tag that covalently interacts with specific substrates. It enables specific covalent labeling of any protein of interest. For more details, see the labeling procedure.

    gpcr-how-it-works-step-1-receptor-labeling-chemical-reaction-c1su1cxcr4
    Step 2 - Understand the assay principle

    Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.

    gpcr-how-it-works-step-2-understand-the-assay-principle-receptor-binding-c1su1cxcr4
    Step 3 - Saturation binding (KD)

    A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells, and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.

    gpcr-how-it-works-step-3-saturation-binding-kd-c1su1cxcr4
    how-it-works-chemokine-cxcr4-cell-line-hek293
    Step 4 - Competitive binding (KI)

    A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.

    gpcr-how-it-works-step-4-competitive-binding-ki-c1su1cxcr4
    how-it-works-chemokine-cxcr4-cell-line-hek293

     

  • Examples of data obtained using chemokine CXCR4-R expressing stable cells (HEK) labelled with terbium in Tag-lite buffer (LABMED), and their matching fluorescent ligand (L0012RED). AMD-3100 was used as reference ligand. Results may vary from one HTRF® compatible reader to another.

    assay-validation-tag-lite-cxcr4-cell-line
    assay-validation-tag-lite-cxcr4-cell-line-2

     

Resources

1-1 of 1 Resources
Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.