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HTRF Strep-Tactin-Eu, 5,000 Assay Points
HTRF Strep-Tactin-Eu, 5,000 Assay Points
HTRF Strep-Tactin-Eu
HTRF Strep-Tactin®-Eu cryptate can be used to capture Strep-tag® II and Twin Strep-tag®-tagged proteins.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Protein-Protein Interaction |
HTRF Strep-Tactin®-Eu cryptate can be used to capture Strep-tag® II and Twin Strep-tag®-tagged proteins.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Product Variants
Unit Size: 50,000 Assay Points
Part #:
610STKLA
List Price
USD 532.89
Unit Size: 500 Assay Points
Part #:
610STKLB
List Price
USD 1,629.00
HTRF Strep-Tactin-Eu, 5,000 Assay Points
HTRF Strep-Tactin-Eu
HTRF Strep-Tactin-Eu, 5,000 Assay Points
Product information
Overview
Strep-Tactin ® has been labeled with Eu crypate. Biotin, Twin Strep-tag ®, and Strep-tag ®II bind to Strep-Tactin ® with high affinity. The binding is rapid and stable, making it an ideal choice for use in a variety of assays such as enzyme assays, protein-protein binding assays, and molecular biology assays.
Specifications
| Application |
Protein-Protein Interaction
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Fluorescent Reagent
|
| Shipping Conditions |
Shipped Ambient
|
| Target Class |
Binding Assay
|
| Technology |
TR-FRET
|
| Unit Size |
50,000 Assay Points
|
Video gallery
HTRF Strep-Tactin-Eu, 5,000 Assay Points
HTRF Strep-Tactin-Eu, 5,000 Assay Points
How it works
Assay principle
In an HTRF interaction assay, one partner is labeled (directly or indirectly) with the donor, and the other with the acceptor (again, directly or indirectly). The intensity of the signal is proportional to the binding of the 2 partners. In the example shown here, Strep-Tactin®-Eu binds to the Strep-Tag®II tagged partner A, while partner B* binds to a specific Ab labeled with an HTRF acceptor. *partner B can also be biotinylated, tagged, Fc fused. In these cases, use the corresponding HTRF reagent (anti-Tag, anti-species, protA, Streptavidin) labeled with an acceptor for the detection.
Assay protocol
The example on the right describes the protocol using a 20 µL final assay volume for detecting an interaction between a Strep-Tag®II-tagged partner A and a non-tagged partner B*. Dispense the 2 partners (10 µL), incubate, add Strep-Tactin®-Eu cryptate (5 µL) and anti-partner B labeled with acceptor (5 µL), incubate, and then read. *partner B can also be tagged, Fc fused or directly labeled. In these cases use the corresponding HTRF reagent (anti-Tag, anti-species, protA, Streptavidin) labeled with acceptor for the detection.
Assay details
How do the number of tests relate to the active moiety?
The average conjugate quantity per well reflects overall biological material content. Using the active moiety amount is generally preferred to as the total quantity of conjugate. For Cryptate and d2 conjugates, the total conjugate amount equals that of the active moiety, since the molecular weight of the label is negligible. This is not the case for XL665 labeled entities, for which the total quantity of conjugate will vary depending on the final molar ratio of the XL665 conjugate. However, the amount of active moiety provided by Cisbio is constant and based on the number of tests ordered.
Recommended quantities of Cryptate and XL665 conjugates
Cryptate conjugates must not be excessive, in order to prevent reader saturation and an unacceptable level of background. In most cases, a cryptate concentration of 1 to 5nM is appropriate, and will generate 20,000 to 80,000 cps at 620 nm depending on the HTRF compatible reader used. The XL665 conjugate must match its assay counterpart as closely as possible in order for the maximum number of biomolecules to be tagged with the XL665 acceptor. Thus, to detect a tagged molecule at an assay concentration of 20nM, the concentration of anti-Tag-XL665 should be equimolar or higher.
Resources
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