Skip to main content
Menu
US

HTRF Strep-Tactin-Eu, 5,000 Assay Points

HTRF Strep-Tactin®-Eu cryptate can be used to capture Strep-tag® II and Twin Strep-tag®-tagged proteins.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW when you buy online.
Promo code: REV15NOW
Offer valid until 11/6. Terms and conditions apply.
Product Variants
Part number: 610STKLA
Unit Size: 50,000 Assay Points
List price: USD 532.89
Your price: Login to view
USD 532.89
USD 532.89 /each
Part number: 610STKLB
Unit Size: 500 Assay Points
List price: USD 1,629.00
Your price: Login to view
USD 0.00
USD 1,629.00 /each

Overview

Strep-Tactin ® has been labeled with Eu crypate. Biotin, Twin Strep-tag ®, and Strep-tag ®II bind to Strep-Tactin ® with high affinity. The binding is rapid and stable, making it an ideal choice for use in a variety of assays such as enzyme assays, protein-protein binding assays, and molecular biology assays.

Specifications

Application
Protein-Protein Interaction
Assay Target Class
Binding Assay
Assay Technology
TR-FRET
Brand
HTRF
Detection Method
HTRF
Product Group
Fluorescent Reagent
Shipping Conditions
Shipped Ambient
Unit Size
50,000 Assay Points

Video gallery

How it works

Assay principle

In an HTRF interaction assay, one partner is labeled (directly or indirectly) with the donor, and the other with the acceptor (again, directly or indirectly). The intensity of the signal is proportional to the binding of the 2 partners. In the example shown here, Strep-Tactin®-Eu binds to the Strep-Tag®II tagged partner A, while partner B* binds to a specific Ab labeled with an HTRF acceptor. *partner B can also be biotinylated, tagged, Fc fused. In these cases, use the corresponding HTRF reagent (anti-Tag, anti-species, protA, Streptavidin) labeled with an acceptor for the detection.

HTRF Strep-Tactin-d2 1b.svg

 

Assay protocol

The example on the right describes the protocol using a 20 µL final assay volume for detecting an interaction between a Strep-Tag®II-tagged partner A and a non-tagged partner B*. Dispense the 2 partners (10 µL), incubate, add Strep-Tactin®-Eu cryptate (5 µL) and anti-partner B labeled with acceptor (5 µL), incubate, and then read. *partner B can also be tagged, Fc fused or directly labeled. In these cases use the corresponding HTRF reagent (anti-Tag, anti-species, protA, Streptavidin) labeled with acceptor for the detection.

HTRF Strep-Tactin-d2 2b.svg

 

Assay details

How do the number of tests relate to the active moiety?

The average conjugate quantity per well reflects overall biological material content. Using the active moiety amount is generally preferred to as the total quantity of conjugate. For Cryptate and d2 conjugates, the total conjugate amount equals that of the active moiety, since the molecular weight of the label is negligible. This is not the case for XL665 labeled entities, for which the total quantity of conjugate will vary depending on the final molar ratio of the XL665 conjugate. However, the amount of active moiety provided by Cisbio is constant and based on the number of tests ordered.
 

HTRF Strep-Tactin-d2 3b.svg

 

Recommended quantities of Cryptate and XL665 conjugates

Cryptate conjugates must not be excessive, in order to prevent reader saturation and an unacceptable level of background. In most cases, a cryptate concentration of 1 to 5nM is appropriate, and will generate 20,000 to 80,000 cps at 620 nm depending on the HTRF compatible reader used. The XL665 conjugate must match its assay counterpart as closely as possible in order for the maximum number of biomolecules to be tagged with the XL665 acceptor. Thus, to detect a tagged molecule at an assay concentration of 20nM, the concentration of anti-Tag-XL665 should be equimolar or higher.

Resources

1-2 of 2 Resources
Brochure Icon
Brochure
HTRF assays and reagents catalog

Discover the versatility and precision of Homogeneous Time-Resolved Fluorescence (HTRF) technology. Our HTRF portfolio offers a...

Guide Icon
Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.

SDS, COAs, Manuals and more

Are you looking for technical documents related to the product? We have categorized them in dedicated sections below. Explore now.

Safety data sheet
+ Show more
Certificate of analysis
+ Show more
Technical data sheet
+ Show more
IFUs, Manuals & more
Scroll Icon