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HTRF Human Phospho-SHP1 (Tyr564) Detection Kit, 10,000 Assay Points

The phospho-SHP1 (Tyr564) kit enables the cell-based quantitative detection of phosphorylated SHP1 as a result of cell surface receptor activation, especially checkpoint inhibitor receptors in T cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: 64SH1PEH
Unit Size: 10,000 Assay Points
Part Number: 64SH1PEG
Unit Size: 500 Assay Points
  • This cell-based assay is designed to monitor the phosphorylation of SHP1 on Tyr564, which represents a hallmark of its activation.

    Many cancer cells overexpress checkpoint inhibitor ligands, such as PD-L1. PD-L1 binds its counterpart checkpoint inhibitor receptor PD1, present at the surface of T lymphocytes. In turn, the PD1-PDL1 complex recruits and activates inhibitory effectors, such as SHP1 or SHP2. These two phosphatases, which are phosphorylated on Tyr564 and Tyr542 respectively by the kinase Lck, trigger the dephosphorylation of signaling proteins such as ZAP-70 or SLP-76, involved in the T cell activation pathway. Finally, activated SHP1 and SHP2 participate in T cell inactivation.

    Preventing the activation of SHP1 and/or SHP2 by small molecule inhibitors is believed to contribute to restoring the immune response against tumors.

  • Assay Points
    10000
    Assay Target Type
    Kit
    Assay Technology
    HTRF
    Therapeutic Area
    Infectious Diseases
    Oncology & Inflammation
    Brand
    HTRF
    Quantity
    1
    Unit Size
    10,000 Assay Points

Resources

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