Skip to main content

HTRF SARS-CoV-2 S1/ACE2 Binding Kit, 500 Assay Points

The SARS-CoV-2 S1/ACE2 binding kit is designed to identify inhibitor or S1/ACE2 interactions.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: 64BDS1PEG
Unit Size: 500 Assay Points
Part Number: 64BDS1PEH
Unit Size: 10,000 Assay Points
  • ACE2 is a human cell surface receptor that has been identified as the main point of entry for SARS-CoV-2 virions, which match and bind ACE2 with their own viral Spike protein S1 subunits. This interaction is therefore a key target in COVID-19 therapeutic research, as its inhibitors may yield a protective effect by preventing SARS-CoV-2 entry into cells. For this reason, protein-protein interaction assays suitable for ACE2-Spike inhibitor screening are relevant to current research.

  • Assay Points
    500
    Assay Target Type
    Kit
    Assay Technology
    HTRF
    Brand
    HTRF
    Quantity
    1
    Shipping Conditions
    Shipped in Dry Ice
    Therapeutic Area
    Infectious Diseases
    Unit Size
    500 Assay Points
  • Assay principle

    The PPI S1/ACE2 binding is mimicking the binding of the Spike S1 protein of SARS-CoV-2 to the Angiotensin Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. This kit should help to identify drugs or antibodies which could interfere or prevent from SARS-CoV-2 infection.

    SARS-CoV-2-S1-ACE2-binding-kit-1.svg
    Assay protocol

    4 µL of the sample is added to a 384 LV volume plate. The sample could be a protein, an Ab or a potential drug. 8 µL of labeled S1 with d2 and 8µl of ACE2 labeled with cryptate are added to the same well. The plate is incubated 24h at +4°C before reding on a specific time resolved fluorescence reader.

    SARS-CoV-2-S1-ACE2-binding-kit-2.svg

     

  • Validation of S1/ACE2 binding kit with unlabeled S1 and ACE2 proteins

    4 µL of the unlabeled S1 or ACE2 proteins were dispensed into a 384 LV volume plate. 8 µL of labeled S1 with d2 and 8µl of ACE2 labeled with cryptate were added to the same wells. The plate was incubated 24h at +4°C before reading on a special time-resolved fluorescence reader.

    SARS-CoV-2-S1-ACE2-binding-kit-3.svg

     

    Validation of S1/ACE2 binding kit with neutralizing Ab control

    4 µL of the neutralizing Ab were added to a 384 LV volume plate. 8 µL of S1 labeled with d2 and 8µl of ACE2 labeled with cryptate were added to the same wells. The plate was incubated 24h at +4°C before reading on a special time-resolved fluorescence reader.

    SARS-CoV-2-S1-ACE2-binding-kit-4.svg

Resources

1-1 of 1 Resources
Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.