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HTRF SARS-CoV2 S1 / ACE2 Binding Kit, 500 Assay Points

The SARS-CoV-2 S1/ACE2 binding kit is designed to identify inhibitor or S1/ACE2 interactions.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Protein-Protein Interaction
Sample Volume 4 µL

The SARS-CoV-2 S1/ACE2 binding kit is designed to identify inhibitor or S1/ACE2 interactions.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 500 Assay Points
Part #:
64BDS1PEG
List Price
USD 2,130.00
Unit Size: 10,000 Assay Points
Part #:
64BDS1PEH
List Price
USD 25,500.00

Overview

ACE2 is a human cell surface receptor that has been identified as the main point of entry for SARS-CoV-2 virions, which match and bind ACE2 with their own viral Spike protein S1 subunits. This interaction is therefore a key target in COVID-19 therapeutic research, as its inhibitors may yield a protective effect by preventing SARS-CoV-2 entry into cells. For this reason, protein-protein interaction assays suitable for ACE2-Spike inhibitor screening are relevant to current research.

Specifications

Application
Protein-Protein Interaction
Brand
HTRF
Detection Modality
HTRF
Product Group
Kit
Sample Volume
4 µL
Shipping Conditions
Shipped in Dry Ice
Target Class
Binding Assay
Technology
TR-FRET
Therapeutic Area
Infectious Diseases
Unit Size
500 Assay Points

Video gallery

How it works

Assay principle

The PPI S1/ACE2 binding is mimicking the binding of the Spike S1 protein of SARS-CoV-2 to the Angiotensin Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. This kit should help to identify drugs or antibodies which could interfere or prevent from SARS-CoV-2 infection.

SARS-CoV-2-S1-ACE2-binding-kit-1.svg
Assay protocol

4 µL of the sample is added to a 384 LV volume plate. The sample could be a protein, an Ab or a potential drug. 8 µL of labeled S1 with d2 and 8µl of ACE2 labeled with cryptate are added to the same well. The plate is incubated 24h at +4°C before reding on a specific time resolved fluorescence reader.

SARS-CoV-2-S1-ACE2-binding-kit-2.svg

 

Assay validation

Validation of S1/ACE2 binding kit with unlabeled S1 and ACE2 proteins

4 µL of the unlabeled S1 or ACE2 proteins were dispensed into a 384 LV volume plate. 8 µL of labeled S1 with d2 and 8µl of ACE2 labeled with cryptate were added to the same wells. The plate was incubated 24h at +4°C before reading on a special time-resolved fluorescence reader.

SARS-CoV-2-S1-ACE2-binding-kit-3.svg

 

Validation of S1/ACE2 binding kit with neutralizing Ab control

4 µL of the neutralizing Ab were added to a 384 LV volume plate. 8 µL of S1 labeled with d2 and 8µl of ACE2 labeled with cryptate were added to the same wells. The plate was incubated 24h at +4°C before reading on a special time-resolved fluorescence reader.

SARS-CoV-2-S1-ACE2-binding-kit-4.svg

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